Radioassay for cobalamin (vitamin B12) requiring no pretreatment of serum

Journal Article · Sat Jan 31 23:00:00 EST 1981 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5925868

Houts, T M; Carney, J A

Researchers describe a radioassay for cobalamin in human serum or plasma that requires no boiling or other pretreatment of the sample. Normal chicken serum covalently coupled to magnetizable particles is used as the binding agent. The assay is performed at pH 12.9, at which pH all cobalamin in human serum is released from its binding proteins, whereas the binding agent maintains a high affinity for cobalamin. Under these assay conditions the binding protein shows a specificity for cobalamin similar to that of purified intrinsic factor. The assay is simple, rapid, and precise, and results correlate well with those of the Euglena gracilis microbiological assay and an intrinsic-factor binding assay.

• OSTI ID: 5925868
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 27:2; ISSN CLCHA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
BLOOD SERUM
CARBOHYDRATES
CHEMICAL PREPARATION
DRUGS
HEMATINICS
HEMATOLOGIC AGENTS
INTRINSIC FACTOR
LABELLED COMPOUNDS
MUCOPROTEINS
ORGANIC COMPOUNDS
PH VALUE
POLYSACCHARIDES
PROTEINS
RADIOASSAY
SACCHARIDES
SAMPLE PREPARATION
SYNTHESIS
VITAMIN B GROUP
VITAMIN B-12
VITAMINS