Processing of newly synthesized cachectin/tumor necrosis factor in endotoxin-stimulate macrophages
- Rockefeller Univ., New York, NY (USA)
The biosynthesis and processing of cachetin/tumor necrosis factor (TNF) were examined in the murine macrophage-like cell line RAW 264.7. Lipipolysaccharide-stimulated cells secreted both glycosylated and nonglycosylated 17-kilodalton (kDa) mature cachectin/TNF into the culture medium. Secreted cachectin/TNF was derived from membrane-associated precursors that were precipitated by polyclonal antisera raised against either the mature protein or synthetic peptide fragments of the 79 amino acid cachectin/TNF prohormone sequence. About half of the precursors were N-glycosylated, apparently cotranslationally. The cachectin/TNF precursors were then proteolytically cleaved to release soluble mature cytokine into the medium, while the membrane-bound 14-kDa prosequence remained cell associated. During the period of LPS stimulation, the amount of macrophage cell surface cachectin/TNF remained at a low level, suggesting that both nonglycosylated and glycosylated precursors of cachectin/TNF are efficiently cleaved by these cells. These findings suggest the presence of a unique mechanism for the secretion of cachectin/TNF.
- OSTI ID:
- 5922579
- Journal Information:
- Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 29:36; ISSN 0006-2960; ISSN BICHA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Identification and characterization of a membrane-bound cytotoxin of murine cytolytic lymphocytes that is related to tumor necrosis factor/cachectin
Studies on the role of glycosylation for human corticosteroid-binding globulin: Comparison with that for thyroxine-binding globulin
Tumor necrosis factor and interleukin 1 as mediators of endotoxin-induced beneficial effects
Journal Article
·
Mon May 01 00:00:00 EDT 1989
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
·
OSTI ID:5161239
Studies on the role of glycosylation for human corticosteroid-binding globulin: Comparison with that for thyroxine-binding globulin
Journal Article
·
Fri Sep 01 00:00:00 EDT 1989
· Endocrinology; (USA)
·
OSTI ID:5607609
Tumor necrosis factor and interleukin 1 as mediators of endotoxin-induced beneficial effects
Journal Article
·
Tue Sep 01 00:00:00 EDT 1987
· Rev. Infect. Dis.; (United States)
·
OSTI ID:5650051
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARBOHYDRATES
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
FIBROBLASTS
ISOTOPES
LIGHT NUCLEI
LIPIDS
LIPOPOLYSACCHARIDES
LIPOTROPIC FACTORS
MACROPHAGES
MAMMALS
MATERIALS
METHIONINE
MICE
NECROSIS
NEOPLASMS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PATHOLOGICAL CHANGES
PEPTIDES
PHAGOCYTES
POLYPEPTIDES
POLYSACCHARIDES
PROTEINS
RADIOISOTOPES
RODENTS
SACCHARIDES
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TOXIC MATERIALS
TOXINS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARBOHYDRATES
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
FIBROBLASTS
ISOTOPES
LIGHT NUCLEI
LIPIDS
LIPOPOLYSACCHARIDES
LIPOTROPIC FACTORS
MACROPHAGES
MAMMALS
MATERIALS
METHIONINE
MICE
NECROSIS
NEOPLASMS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PATHOLOGICAL CHANGES
PEPTIDES
PHAGOCYTES
POLYPEPTIDES
POLYSACCHARIDES
PROTEINS
RADIOISOTOPES
RODENTS
SACCHARIDES
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TOXIC MATERIALS
TOXINS
VERTEBRATES