Sequential sup 1 H NMR assignments and secondary structure of the B domain of staphylococcal protein A: Structural changes between the free B domain in solution and the Fc-bound B domain in crystal

Journal Article · Tue Sep 18 04:00:00 EDT 1990 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00489a040· OSTI ID:5920959

Torigoe, Hidetaka; Shimada, Ichio; Arata, Yoji ^[1]; Saito, Akiko; Sato, Moriyuki ^[2]

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1. Univ. of Tokyo (Japan)
2. Kyowa Hakko Kogyo Co., Asahimachi, Tokyo (Japan)

The recombinant B domain (FB) of staphylococcal protein A, which specifically binds to the Fc portion of immunoglobulin G (IgG), has been investigated with the use of two-dimensional proton nuclear magnetic resonance spectroscopy. All backbone and side-chain proton resonances of FB (60 amino acid residues), except the amide proton resonance of Ala2, were assigned by the sequential assignment procedures by using double-quantum-filtered correlated spectroscopy (DQF-COSY), homonuclear Hartmann-Hahn spectroscopy (HOHAHA), and nuclear Overhauser enhancement spectroscopy (NOESY). On the basis of the NOESY data, three helical regions, Glu9-His19, Glu25-Asp37, and Ser42-Ala55, were identified in the free FB in solution. Existence of two of the three helical regions, Glu9-His19 and Glu25-Asp37, is consistent with the X-ray crystallographic structure of the Fc-bound FB. By contrast, in the Fc-bound FB as revealed by the X-ray analysis, the Ser42-Glu48 segment and no structural information has been available in the Ala49-Ala55 segment. The authors suggest that a significant conformation change is induced in the C-terminal region of FB when it is bound to the Fc portion of IgG.

• OSTI ID: 5920959
• Journal Information: Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 29:37; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
AZOLES
BACTERIA
BARYONS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL WALL
CHEMICAL SHIFT
CONFORMATIONAL CHANGES
ELEMENTARY PARTICLES
FERMIONS
GLUTAMIC ACID
HADRONS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HISTIDINE
HYDROXY ACIDS
IMIDAZOLES
MAGNETIC RESONANCE
MICROORGANISMS
NUCLEAR MAGNETIC RESONANCE
NUCLEONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OVERHAUSER EFFECT
PROTEINS
PROTONS
RESONANCE
SERINE
STAPHYLOCOCCUS
TEMPERATURE DEPENDENCE