NMR signal assignments of amide protons in the. cap alpha. -helical domains of staphylococcal nuclease
The authors report complete assignments of the amide proton signals in the three long d/sub NN/ connectivity sequences observed in the NOESY spectrum of deuteriated staphylococcal nuclease (Nase) complexed with thymidine 3',5'-bisphosphate (pdTp) and Ca/sup 2 +/, M/sub r/ 18K. The assignments are made by comparing NOESY spectra with /sup 1/H-/sup 15/N and /sup 1/H-/sup 13/C heteronuclear multiple-quantum shift correlation (HMQC) spectra of Nase samples containing /sup 15/N- and /sup 13/C-labeled amino acids. The assignments show that the residues which are linked by the d/sub NN/ connectivity sequences are located in the three ..cap alpha..-helical domains of Nase. The results indicate that by combining NOESY and HMQC spectra of appropriately labeled samples it should be possible to delineate and study ..cap alpha..-helical domains in soluble proteins having molecular weights that are greater than 18K.
- Research Organization:
- National Institutes of Health, Bethesda, MD (USA)
- OSTI ID:
- 6901490
- Journal Information:
- Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 27:14; ISSN BICHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALINE EARTH METAL COMPOUNDS
AMINO ACID SEQUENCE
AMINO ACIDS
BACTERIA
BARYONS
CALCIUM COMPOUNDS
CARBON 13
CARBON ISOTOPES
CARBOXYLIC ACIDS
CATIONS
CHARGED PARTICLES
CHEMICAL SHIFT
ELEMENTARY PARTICLES
ENZYMES
ESCHERICHIA COLI
ESTERASES
EVEN-ODD NUCLEI
FERMIONS
HADRONS
HYDROLASES
IONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MICROORGANISMS
MOLECULAR STRUCTURE
MOLECULAR WEIGHT
NITROGEN 15
NITROGEN ISOTOPES
NUCLEAR MAGNETIC RESONANCE
NUCLEASES
NUCLEI
NUCLEONS
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
OVERHAUSER EFFECT
PHOSPHODIESTERASES
PROTONS
RESONANCE
STABLE ISOTOPES
STAPHYLOCOCCUS