Studies on the biological effects of ozone: 2. Induction of tumor necrosis factor (TNF-alpha) on human leucocytes
Journal Article
·
· Lymphokine Cytokine Research; (United States)
OSTI ID:5905831
- Institute of General Physiology, University of Siena (Italy)
The effect of ozone as a probable inducer of tumor necrosis factor (TNF-alpha) has been investigated on human blood and on Ficoll-purified blood mononuclear cells (PBMC). Samples were exposed at different ozone concentrations ranging from 2.2 to 108 micrograms/ml and incubated at 37 degrees C in an 95% air-5% CO2 atmosphere. At predetermined times, all cell supernatants were tested for TNF activity and some PBMC cultures were examined for DNA synthesis. The authors have shown that ozone concentration is critical in terms of TNF production and of cell mitogenesis and that, owing to the presence of erythrocytes, higher ozone concentrations are required to be effective in blood than in PBMC. Because ozonization of blood is a procedure followed in several European countries for the treatment of viral diseases and tumors, the release of factors with antiviral and immunomodulatory activities by leukocytes may explain the mechanism of action of ozone and of autohemotherapy.
- OSTI ID:
- 5905831
- Journal Information:
- Lymphokine Cytokine Research; (United States), Journal Name: Lymphokine Cytokine Research; (United States) Vol. 10:5; ISSN 1056-5477; ISSN XZ061
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL DIVISION
DNA REPLICATION
ENZYME IMMUNOASSAY
GROWTH FACTORS
IMMUNOASSAY
LEUKOCYTES
LYMPHOKINES
MAMMALS
MAN
MATERIALS
MITOGENS
MITOSIS
MONOCYTES
NUCLEIC ACID REPLICATION
ORGANIC COMPOUNDS
OZONE
PRIMATES
PROTEINS
SYNTHESIS
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL DIVISION
DNA REPLICATION
ENZYME IMMUNOASSAY
GROWTH FACTORS
IMMUNOASSAY
LEUKOCYTES
LYMPHOKINES
MAMMALS
MAN
MATERIALS
MITOGENS
MITOSIS
MONOCYTES
NUCLEIC ACID REPLICATION
ORGANIC COMPOUNDS
OZONE
PRIMATES
PROTEINS
SYNTHESIS
VERTEBRATES