Glutathione (GSH) depletion: Effect on chromate (Cr)-induced alterations of renal plasma membrane transport
- Univ. of Nebraska, Omaha (United States)
In this study, renal membrane vesicles were isolated from control Sprague Dawley rats, and those treated with DEM DEM+BSO, DEM+Cr and DEM+BSO+Cr. GSH depletors were given 45 min before Cr and the rats sacrificed 3 hr later. Renal GSH was measured and membrane vesicles isolated by Percoll gradient centrifugation. GSH depletion by DEM alone had no effect on BL PAH transport or BB glucose transport. PAH overshoot was absent when Cr was given after DEM, as was true when Cr was given alone, and glucose transport was reduced modestly. GSH depletion by DEM+BSO did not markedly alter either PAH or glucose transport. When Cr was administered after DEM+BSO, the PAH overshoot was eliminated completely, and a reduced glucose transport was seen compared to Cr-DEM and Cr alone. These data suggest that the greater GSH depletion with DEM+BSO permitted an effect of Cr not seen in controls or DEM treated rats.
- OSTI ID:
- 5905003
- Report Number(s):
- CONF-9104107--
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 5:4; ISSN 0892-6638; ISSN FAJOE
- Country of Publication:
- United States
- Language:
- English
Similar Records
Cellular glutathione depletion by diethyl maleate or buthionine sulfoximine: no effect of glutathione depletion on the oxygen enhancement ratio
Depletion of glutathione in vivo as a method of improving the therapeutic ratio of misonidazole and SR 2508. [BSO; DEM]
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
AMINO ACIDS
ANIMALS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CENTRIFUGATION
CHROMATES
CHROMIUM COMPOUNDS
DRUGS
GLUCOSE
GLUTATHIONE
HEXOSES
HIPPURIC ACID
KIDNEYS
MAMMALS
MEMBRANE TRANSPORT
METABOLISM
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RATS
RODENTS
SACCHARIDES
SEPARATION PROCESSES
SYNTHESIS
TRANSITION ELEMENT COMPOUNDS
ULTRACENTRIFUGATION
VERTEBRATES