Characterization of the high-affinity cell-surface receptor for murine B-cell-stimulating factor 1
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
Radiolabeled recombinant murine B-cell-stimulatory factory 1 (BSF-1) was used to characterize receptors specific for this lymphokine on the surface of primary B and T cells and in vitro cell lines representing the B-cell, T-cell, mast cell, macrophage, and myelomonocytic lineages. BSF-1 binding was rapid and saturable at 4/sup 0/C and 37/sup 0/C with a slow dissociation rate. On all cell types examined, BSF-1 bound to a single class of high-affinity receptor (<20000 receptors per cell) with a K/sub a/ of 10/sup 10/-10/sup 11/ M/sup -1/. Receptor expression on resting primary B and T cells was low (<100 receptors per cell), whereas activation with lipopolysaccharide or Con A produced a 5- to 10-fold increase in receptor numbers. Among a panel of lymphokines and growth hormones, only unlabeled BSF-1 was able to compete for the binding of /sup 125/I-labeled BSF-1. Affinity crosslinking experiments resulted in the identification on all cells tested on a receptor protein with an average M/sub r/ of 75,000.
- Research Organization:
- Immunex Corp., Seattle, WA
- OSTI ID:
- 5903526
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:6; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CHEMICAL REACTIONS
CONFIGURATION INTERACTION
CONNECTIVE TISSUE CELLS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ESTERS
GROWTH FACTORS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MICE
MITOGENS
MOLECULAR STRUCTURE
MOLECULAR WEIGHT
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PHORBOL ESTERS
POLYMERIZATION
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RECEPTORS
RODENTS
SOMATIC CELLS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CHEMICAL REACTIONS
CONFIGURATION INTERACTION
CONNECTIVE TISSUE CELLS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ESTERS
GROWTH FACTORS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MICE
MITOGENS
MOLECULAR STRUCTURE
MOLECULAR WEIGHT
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PHORBOL ESTERS
POLYMERIZATION
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RECEPTORS
RODENTS
SOMATIC CELLS
VERTEBRATES