Characterization of cell-surface receptors for monoclonal-nonspecific suppressor factor (MNSF)

Journal Article · Mon Oct 15 04:00:00 EDT 1990 · Cellular Immunology; (USA)

DOI:https://doi.org/10.1016/0008-8749(90)90271-R· OSTI ID:6074343

Nakamura, M; Ogawa, H; Tsunematsu, T ^[1]

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1. Shimane Medical Univ., Izumo (Japan)

Monoclonal-nonspecific suppressor factor (MNSF) is a lymphokine derived from murine T cell hybridoma. The target tissues are both LPS-stimulated B cells and Con A-stimulated T cells. Since the action of MNSF may be mediated by its binding to specific cell surface receptors, we characterized the mode of this binding. The purified MNSF was labeled with {sup 125}I, using the Bolton-Hunter reagent. The labeled MNSF bound specifically to a single class of receptor (300 receptors per cell) on mitogen-stimulated murine B cells or T cells with an affinity of 16 pM at 24{degrees}C, in the presence of sodium azide. Competitive experiments showed that MNSF bound to the specific receptor and that the binding was not shared with IL2, IFN-gamma, and TNF. Various cell types were surveyed for the capacity to specifically bind {sup 125}I-MNSF. {sup 125}I-MNSF bound to MOPC-31C (a murine plasmacytoma line) and to EL4 (a murine T lymphoma line). The presence of specific binding correlates with the capacity of the cells to respond to MNSF. These data support the view that like other polypeptide hormones, the action of MNSF is mediated by specific cell surface membrane receptor protein. Identification of these receptors will provide insight into the apparently diverse activities of MNSF.

• OSTI ID: 6074343
• Journal Information: Cellular Immunology; (USA), Journal Name: Cellular Immunology; (USA) Vol. 130:2; ISSN 0008-8749; ISSN CLIMB
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOHYDRATES
CHEMICAL COMPOSITION
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GROWTH FACTORS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LEUKOCYTES
LIPIDS
LIPOPOLYSACCHARIDES
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MICE
MITOGENS
MOLECULAR WEIGHT
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
POLYSACCHARIDES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RODENTS
SACCHARIDES
SOMATIC CELLS
SPLEEN
TRACER TECHNIQUES
VERTEBRATES