Binding of /sup 125/I-hCG to rainbow trout (Salmo gairdneri) testis in vitro. [Human Chorionic Gonadotropin]
Homogenates of maturing rainbow trout testes show specific binding sites for /sup 125/I-labeled hCG (. /sup 125/I-labeled hCG). The binding is competitively inhibited by unlabeled hCG and by a hypophyseal extract of rainbow trout. It could be demonstrated that the tissue /sup 125/I-hCG binding specificity is restricted to the gonadal preparation. The trout testis was characterized by determining affinity and capacity from Scatchard plot analysis giving a high constant of dissociation Kd 3.65 x 10(-10)/M and a low binding capacity of 0.88 x 10(-15) M/mg tissue. The test system is markedly dependent on temperature, incubation-time, and pH. The maximum binding was found at 37 degrees during 2 hr of incubation in a buffer of pH 7.5.
- Research Organization:
- Ruhr-Universitaet Bochum, Abteilung Biologie, West Germany
- OSTI ID:
- 5899740
- Journal Information:
- Gen. Comp. Endocrinol.; (United States), Vol. 49:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HCG
CHEMICAL BONDS
RECEPTORS
IODINE 125
LABELLED COMPOUNDS
TESTES
TRACER TECHNIQUES
TROUT
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FISHES
GONADOTROPINS
GONADS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MALE GENITALS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOISOTOPES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques