Increased survival of skin flaps by scavengers of superoxide radical
Elevation of rat abdominal skin flaps, followed by ligation and division of the left inferior neurovascular pedicle, resulted in only a 40% survival of the area normally perfused by the ligated artery and vein. Superoxide dismutase (SOD) (EC1.15.1.1) administered v (20,000 U/kg) 30 min before flap elevation increased survival to 52%. SOD derivatized with polyethylene gycol, which increases circulating half-life, was more effective, increasing survival to 80%. This protective effect resulted from the catalytic activity of the derivatized enzyme because inactivation by treatment with H/sub 2/O/sub 2/ eliminated its effect on skin flap survival. An equimolar mixture of Desferal and MnCl/sub 2/, which catalyzes the dismutation of O/sub 2//sup -/ in vitro, improved survival to 72%. Desferal-Fe/sup 3 +/, which lacks in vitro SOD activity, or Mn/sup 2 +/ alone did not affect the survival of skin flaps, but Desferal alone was nearly as effective as the Desferal-Mn/sup 2 +/ mixture. This effect of Desferal may result from acquisition of and subsequent removal of iron in vivo. These results support the view that the superoxide radical or a product derived from it plays a role in limiting the survival of island skin flaps.
- Research Organization:
- Duke Univ. Medical Center, Durham, NC
- OSTI ID:
- 5898446
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 1:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
SUPEROXIDE DISMUTASE
BIOLOGICAL EFFECTS
SUPEROXIDE RADICALS
SCAVENGING
BIOLOGICAL MODELS
CHELATING AGENTS
IRON COMPOUNDS
ISCHEMIA
PATHOGENESIS
PERFUSED TISSUES
RATS
SKIN
ANIMAL TISSUES
ANIMALS
BODY
CARDIOVASCULAR DISEASES
DISEASES
ENZYMES
MAMMALS
ORGANS
OXIDOREDUCTASES
RADICALS
RODENTS
TISSUES
TRANSITION ELEMENT COMPOUNDS
VASCULAR DISEASES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology