Effects of buthionine sulfoximine on Cd-binding peptide levels in suspension-cultured tobacco cells treated with Cd, Zn, or Cu

Journal Article · Wed Jul 01 00:00:00 EDT 1987 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.84.3.574· OSTI ID:5898345

Inhibition of cell growth and accumulation of Cd-binding peptide were measured in cultured tobacco cells exposed to buthionine sulfoximine. This inhibitor of glutathione metabolism caused little or no reduction of growth (at 0.1 millimolar) in the absence of Cd, but growth was greatly reduced in cultures exposed to buthionine sulfoximine and greater than or equal to 22 micromolar Cd. Decreased cell growth was directly correlated with decreased levels of Cd-binding peptide and increased level of what is thought to be free Cd. Zinc inhibited growth of tobacco cells only at the highest levels examined (900-1800 micromolar Zn), but buthionine sulfoximine had no additional significantly effect. Similar results were observed for Cu (45-90 micromolar). Results suggest that synthesis of plant Cd-peptide involves ..gamma..-glutamylcysteine synthetase or a related enzyme and that Zn accumulation in tobacco cells does not cause formation of significant Cd-peptide ligand.

• Research Organization: Univ. of Kentucky, Lexington
• OSTI ID: 5898345
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 84:3; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CADMIUM
CELL CULTURES
COPPER
DRUGS
ELEMENTS
ENZYMES
GLUTATHIONE
GROWTH
INHIBITION
LIGANDS
LIGASES
METABOLISM
METALS
NICOTIANA
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDES
PLANT CELLS
PLANT GROWTH
PLANTS
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
SYNTHESIS
TRANSITION ELEMENTS
ZINC