Effect of glutathione on phytochelatin synthesis in tomato cells. [Lycopersicon esculentum]
- Purdue Univ., West Lafayette, IN (USA)
Growth of cell suspension cultures of tomato, Lycopersicon esculentum Mill. cv VFNT-Cherry, in the presence of cadmium is inhibited by buthionine sulfoximine, an inhibitor of glutathione synthesis. Cell growth and phytochelatin synthesis are restored to cells treated with buthionine sulfoximine by the addition of glutathione to the medium. Glutathione stimulates the accumulation of phytochelatins in cadmium treated cells, indicating that availability of glutathione can limit synthesis of these peptides. Exogenous glutathione causes a disproportionate increase in the level of smaller phytochelatins, notably ({gamma}-Glu-Cys){sub 2}-Gly. In the presence of buthionine sulfoximine and glutathione, phytochelatins that are produced upon exposure to cadmium incorporate little ({sup 35}S)cysteine, indicating that these peptides are probably not synthesized by sequential addition of cysteine and glutamate to glutathione.
- OSTI ID:
- 5852811
- Journal Information:
- Plant Physiology; (USA), Vol. 93:2; ISSN 0032-0889
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CADMIUM
BIOLOGICAL EFFECTS
GLUTATHIONE
BIOCHEMICAL REACTION KINETICS
TOMATOES
PHYSIOLOGY
CYSTEINE
ENZYME INHIBITORS
PEPTIDES
PLANT CELLS
SULFUR 35
TRACER TECHNIQUES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DRUGS
ELEMENTS
EVEN-ODD NUCLEI
FOOD
FRUITS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
METALS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
REACTION KINETICS
SULFUR ISOTOPES
THIOLS
551001* - Physiological Systems- Tracer Techniques