Phosphorylation of PI-specific phospholipase C by protein kinase C in vivo
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5897882
A phosphoinositide-specific phospholipase C (PI-PLC I) was purified to homogeneity from guinea pig uterus. The purified enzyme migrated as a 62 kDa protein on SDS-polyacrylamide gels. PI-PLC I hydrolyzed all three phosphoinositides exhibiting a greater V/sub max/ for PIP/sub 2/ > PIP > PI. The maximal rate of hydrolysis of PIP and PIP/sub 2/ occurred at 700 nM free calcium. Antibodies specific for PI-PLC I reacted with a 62 kDa protein in membrane and cytosolic fractions from guinea pig uterus. The membrane associated PI-PLC I was tightly bound to the membrane; requiring detergent extraction for complete solubilization. The membrane bound form of PI-PLC I failed to bind lectin affinity columns, suggesting that it was not glycosylated. Using monospecific antibodies, it was demonstrated that PI-PLC I was phosphorylated in vivo. Treatment of rat basophilic leukemic cells with active phorbol esters resulted in a time-dependent increase in the phosphorylation of PI-PLC I. The phosphorylated form of PI-PLC I was found in the cytosolic fraction of untreated cells and in both cytosolic and membrane fractions of cells treated with active phorbol esters. These results suggest that protein kinase C may play an important role in the regulation of phospholipase C in vivo.
- Research Organization:
- Smith Kline and French Labs., Philadelphia, PA
- OSTI ID:
- 5897882
- Report Number(s):
- CONF-870644-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BODY
CARBOXYLESTERASES
CARCINOGENS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
ELECTROPHORESIS
ENZYMES
ESTERASES
ESTERS
FEMALE GENITALS
FUNCTIONS
GUINEA PIGS
HYDROLASES
KINETICS
LIPASE
MAMMALS
MEMBRANES
ORGANIC COMPOUNDS
ORGANS
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
RATS
REACTION KINETICS
RODENTS
TRANSFERASES
TUMOR CELLS
UTERUS
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BODY
CARBOXYLESTERASES
CARCINOGENS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
ELECTROPHORESIS
ENZYMES
ESTERASES
ESTERS
FEMALE GENITALS
FUNCTIONS
GUINEA PIGS
HYDROLASES
KINETICS
LIPASE
MAMMALS
MEMBRANES
ORGANIC COMPOUNDS
ORGANS
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
RATS
REACTION KINETICS
RODENTS
TRANSFERASES
TUMOR CELLS
UTERUS
VERTEBRATES