Molecular cloning and expression of Corynebacterium glutamicum genes for amino acid synthesis in Escherichia coli cells
Molecular cloning of Corynebacterium glutamicum genes for threonine and lysine synthesis has been done in Escherichia coli cells. The clonal library of EcoRI fragments of chromosomal DNA of C. glutamicum was constructed on the plasmid vector /lambda/pSL5. The genes for threonine and lysine synthesis were identified by complementation of E. coli mutations in thrB and lysA genes, respectively. Recombinant plasmids, isolated from independent ThrB/sup +/ clone have a common 4.1-kb long EcoRI DNA fragment. Hybrid plasmids isolated from LysA/sup +/ transductants of E. coli have common 2.2 and 3.3 kb long EcoRI fragments of C. glutamicum DNA. The hybrid plasmids consistently transduced the markers thrB/sup +/ and lysA/sup +/. The Southern hybridization analysis showed that the cloned DNA fragments hybridized with the fragments of identical length in C. glutamicum chromosomes.
- Research Organization:
- All-Union Research Institute of Genetics and Breeding of Industrial Microorganisms, Moscow (USSR)
- OSTI ID:
- 5896388
- Journal Information:
- Sov. Genet. (Engl. Transl.); (United States), Vol. 24:7; Other Information: Translated from Genetika; 24: No. 7, 1153-1158(Jul 1988)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ENDONUCLEASES
DNA-CLONING
LIGASES
LYSINE
BIOSYNTHESIS
PLASMIDS
THREONINE
BACTERIA
BACTERIOPHAGES
CHROMOSOMES
CLONE CELLS
DNA SEQUENCING
ELECTROPHORESIS
ESCHERICHIA COLI
GENE MUTATIONS
HYBRIDIZATION
LABELLED COMPOUNDS
MOLECULAR STRUCTURE
PHOSPHORUS 32
RADIOASSAY
TRANSCRIPTION
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL CULTURES
CLONING
DAYS LIVING RADIOISOTOPES
DNA HYBRIDIZATION
DNA-ASE
ENZYMES
ESTERASES
HYDROLASES
HYDROXY ACIDS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
MUTATIONS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PHOSPHODIESTERASES
PHOSPHORUS ISOTOPES
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
VIRUSES
550401* - Genetics- Tracer Techniques
550701 - Microbiology- Tracer Techniques
550301 - Cytology- Tracer Techniques