Molecular cloning and expression of Corynebacterium glutamicum genes for amino acid synthesis in Escherichia coli cells

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Sov. Genet. (Engl. Transl.); (United States)

OSTI ID:5896388

Beskrovnaya, O Yu; Fonshtein, M Yu; Kolibaba, L G; Yankovskii, N K; Debabov, V G

Molecular cloning of Corynebacterium glutamicum genes for threonine and lysine synthesis has been done in Escherichia coli cells. The clonal library of EcoRI fragments of chromosomal DNA of C. glutamicum was constructed on the plasmid vector /lambda/pSL5. The genes for threonine and lysine synthesis were identified by complementation of E. coli mutations in thrB and lysA genes, respectively. Recombinant plasmids, isolated from independent ThrB/sup +/ clone have a common 4.1-kb long EcoRI DNA fragment. Hybrid plasmids isolated from LysA/sup +/ transductants of E. coli have common 2.2 and 3.3 kb long EcoRI fragments of C. glutamicum DNA. The hybrid plasmids consistently transduced the markers thrB/sup +/ and lysA/sup +/. The Southern hybridization analysis showed that the cloned DNA fragments hybridized with the fragments of identical length in C. glutamicum chromosomes.

• Research Organization: All-Union Research Institute of Genetics and Breeding of Industrial Microorganisms, Moscow (USSR)
• OSTI ID: 5896388
• Journal Information: Sov. Genet. (Engl. Transl.); (United States), Journal Name: Sov. Genet. (Engl. Transl.); (United States) Vol. 24:7; ISSN SOGEB
• Country of Publication: United States
• Language: English

Related Subjects

550301 -- Cytology-- Tracer Techniques
550401* -- Genetics-- Tracer Techniques
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
BACTERIA
BACTERIOPHAGES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL CULTURES
CHROMOSOMES
CLONE CELLS
CLONING
DAYS LIVING RADIOISOTOPES
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-ASE
DNA-CLONING
ELECTROPHORESIS
ENDONUCLEASES
ENZYMES
ESCHERICHIA COLI
ESTERASES
GENE MUTATIONS
HYBRIDIZATION
HYDROLASES
HYDROXY ACIDS
ISOTOPES
LABELLED COMPOUNDS
LIGASES
LIGHT NUCLEI
LYSINE
MICROORGANISMS
MOLECULAR STRUCTURE
MUTATIONS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PHOSPHODIESTERASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMIDS
RADIOASSAY
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
THREONINE
TRANSCRIPTION
VIRUSES