Development and validation of rapid in-situ assays of environmental mutagenesis. Annual technical report, 1 Jul 89-30 Sep 90

Technical Report · Wed Oct 31 04:00:00 EST 1990

OSTI ID:5893015

McBee, K

All field work at a site contaminated with polychlorinated biphenyls (PCBs) in Pryor, Oklahoma has been completed. Standard chromosome aberration assays on Peromyscus leucopus (white-footed mouse), Sigmodon hispidus (cotton rat), and Reithroduontomys fulvescens (fulvous harvest mouse) from the Pryor site and from three matched reference sites have been completed. All samples of spleen tissues for flow cytometric analyses (FCM) have been prepared. Trial runs for FCM analysis have been carried out and all FCM analyses should be completed by the end of the year. PCB tissue content analysis has been completed on seven animals randomly chosen from the Pryor sites and shows significant increases over background levels. Field analyses at a second site in Payne Country, Oklahoma contaminated with a mixture of radioactive and chemical wastes has been underway for one year. Data collection has involved monthly sampling for several demographic variables and tri-monthly sampling for cytogenetic and tissue residue variables. Field work for this year has been completed and slides are currently being analyzed. Laboratory validation experiments involving exposure to known levels of known clastogens have been initiated.

• Research Organization: Oklahoma State Univ., Stillwater, OK (United States). Dept. of Zoology
• OSTI ID: 5893015
• Report Number(s): AD-A-243058/5/XAB; CNN: AFOSR-89-0194
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540220* -- Environment
Terrestrial-- Chemicals Monitoring & Transport-- (1990-)
540230 -- Environment
Terrestrial-- Radioactive Materials Monitoring & Transport-- (1990-)
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AROMATICS
BODY
CELL FLOW SYSTEMS
CHEMICAL WASTES
CHLORINATED AROMATIC HYDROCARBONS
CHROMOSOMAL ABERRATIONS
DATA ACQUISITION
DEVELOPED COUNTRIES
FEDERAL REGION VI
HALOGENATED AROMATIC HYDROCARBONS
LAND POLLUTION
MAMMALS
MANAGEMENT
MATERIALS
MICE
MUTAGEN SCREENING
MUTATIONS
NONRADIOACTIVE WASTES
NORTH AMERICA
OKLAHOMA
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANS
POLLUTION
RADIOACTIVE MATERIALS
RADIOACTIVE WASTES
RATS
RODENTS
SAMPLING
SCREENING
SPLEEN
TESTING
USA
VALIDATION
VERTEBRATES
WASTE DISPOSAL
WASTE MANAGEMENT
WASTES