Effect of copper and d-penicillamine treatment on the ocular inflammatory response and plasma antioxidant activity

Conference · Mon Feb 26 04:00:00 EST 1990 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5892169

McGahan, M C; Grimes, A M ^[1]

---

1. North Carolina State Univ., Raleigh (United States)

Copper (Cu) complexes of a number of compounds have increased efficacy against many pathological conditions when compared to the parent compound. In the present study, the authors combined Cu treatment with daily administration of d-penicillamine (d-pen; 100 mg/kg) to determine effectiveness against the ocular inflammatory response to endotoxin. Four groups of rabbits (New Zealand white) were used in this study. Groups 1 and 2 were sham operated and Group 2 received daily injections of d-pen. Groups 3 and 4 had subcutaneously implanted osmotic pumps delivering 4.15 mg Cu per day, with Group 4 also receiving daily injections of d-pen. After 6 days of treatment the eyes were injected intravitreally with 10 ng endotoxin. Twenty-four hours later the eyes were examined, the rabbits killed and plasma and eyes obtained for analysis. There were no differences in the ocular inflammatory response between any of the groups. However, plasma antioxidant activity was significantly greater in Group IV than in any of the other groups. Plasma from groups 1-4 inhibited spontaneous lipid peroxidation by 38.9, 37.4, 54.1 and 69.2%, respectively. Increased plasma antioxidant activity in Group 4 was not due to a Cu/d-pen complex or any other compound with a molecular weight less than 10,000, but could possibly contribute to the increased anti-inflammatory efficacy of Cu/d-pen treatment found in other models of inflammation.

• OSTI ID: 5892169
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 4:3
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMALS
ANTIGENS
ANTIOXIDANTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY
BODY AREAS
BODY FLUIDS
CARBOXYLIC ACIDS
CHELATING AGENTS
CHEMICAL REACTIONS
COMPLEXES
COPPER COMPLEXES
DRUGS
ENDOTOXINS
EYES
FACE
HEAD
INFLAMMATION
LIPIDS
MAMMALS
MATERIALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OXIDATION
PATHOLOGICAL CHANGES
PENICILLAMINE
RABBITS
RADIOPROTECTIVE SUBSTANCES
SENSE ORGANS
SYMPTOMS
THERAPEUTIC USES
THIOLS
TOXIC MATERIALS
TOXINS
TRANSITION ELEMENT COMPLEXES
USES
VERTEBRATES