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Control of dihydrofolate reductase messenger ribonucleic acid production

Journal Article · · Mol. Cell. Biol.; (United States)
DOI:https://doi.org/10.1128/MCB.1.11.961· OSTI ID:5891813
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The authors used methotrexate-resistant mouse cells in which dihydrofolate reductase levels are approximately 500 times normal to study the effect of growth stimulation on dihydrofolate reductase gene expression. As a result of growth stimulation, the relative rate of dihydrofolate reductase protein synthesis increased threefold, reaching a maximum between 25 and 30 h after stimulation. The relative rate of dihydrofolate reductase messenger ribonucleic acid production (i.e., the appearance of dihydrofolate reductase messenger ribonucleic acid in the cytoplasm) increased threefold after growth stimulation and was accompanied by a corresponding increase in the relative steady-state level of dihydrofolate reductase ribonucleic acid in the nucleus. However, the increase in the nuclear level of dihydrofolate reductase ribonucleic acid was not accompanied by a significant increase in the relative rate of transcription of the dihydrofolate reductase genes. These data indicated that the relative rate of appearance of dihydrofolate reductase messenger ribonucleic acid in the cytoplasm depends on the relative stability of the dihydrofolate reductase ribonucleic acid sequences in the nucleus and is not dependent on the relative rate of transcription of the dihydrofolate reductase genes.
Research Organization:
Marrs McLean Dept. of Biochemistry, Baylor College of Medicine, Texas Medical Center, Houston, TX 77030
OSTI ID:
5891813
Journal Information:
Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 1:11; ISSN MCEBD
Country of Publication:
United States
Language:
English

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Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
ANTIMETABOLITES
AROMATICS
AZAARENES
BIOLOGICAL FUNCTIONS
CARBOXYLIC ACIDS
CELL CULTURES
DNA SEQUENCING
DRUGS
ENZYME INDUCTION
ENZYMES
FOLIC ACID
FUNCTIONS
GENE AMPLIFICATION
GENE REGULATION
GENES
HEMATINICS
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
MAMMALS
MESSENGER-RNA
METHOTREXATE
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PHENOTYPE
PTERIDINES
RATS
RNA
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
TOXICITY
TRANSCRIPTION
VERTEBRATES
VITAMIN B GROUP
VITAMINS