Amplification and loss of dihydrofolate reductase genes in a Chinese hamster ovary cell line
Journal Article
·
· Mol. Cell. Biol.; (United States)
During stepwise increases in the methotrexate concentration in culture medium, the authors selected Chinese hamster ovary cells that contained elevated dihydrofolate reductase levels which were proportional to the number of dihydrofolate reductase gene copies (i.e., gene amplification). The authors studied the dihydrofolate reductase levels in individual cells that underwent the initial steps of methotrexate resistance by using the fluorescence-activated cell sorter technique. Such cells constituted a heterogeneous population with differing dihydrofolate reductase levels, and they characteristically lost the elevated enzyme levels when they were grown in the absence of methotrexate. The progeny of individual cells with high enzyme levels behaved differently and could lose all or variable numbers of the amplified genes.
- Research Organization:
- Dept. of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139
- OSTI ID:
- 5877538
- Journal Information:
- Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 1:12; ISSN MCEBD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550400* -- Genetics
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMAL CELLS
ANTIMETABOLITES
AROMATICS
AZAARENES
BIOASSAY
BIOLOGICAL FUNCTIONS
CARBOXYLIC ACIDS
CHO CELLS
CULTURE MEDIA
DRUGS
ENZYMES
FOLIC ACID
FUNCTIONS
GENE AMPLIFICATION
GENES
GROWTH
HEMATINICS
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
METHOTREXATE
MUTAGENESIS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PROGENY
PTERIDINES
RESPONSE MODIFYING FACTORS
TOXICITY
VITAMIN B GROUP
VITAMINS
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMAL CELLS
ANTIMETABOLITES
AROMATICS
AZAARENES
BIOASSAY
BIOLOGICAL FUNCTIONS
CARBOXYLIC ACIDS
CHO CELLS
CULTURE MEDIA
DRUGS
ENZYMES
FOLIC ACID
FUNCTIONS
GENE AMPLIFICATION
GENES
GROWTH
HEMATINICS
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
METHOTREXATE
MUTAGENESIS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PROGENY
PTERIDINES
RESPONSE MODIFYING FACTORS
TOXICITY
VITAMIN B GROUP
VITAMINS