Photosystem II-phycobilisome preparation from the red alga, Porphyridium cruentum: oxygen evolution, ultrastructure, and polypeptide resolution

Journal Article · Mon Apr 01 04:00:00 EST 1985 · Arch. Biochem. Biophys.; (United States)

DOI:https://doi.org/10.1016/0003-9861(85)90135-3· OSTI ID:5882462

Clement-Metral, J D; Gantt, E; Redlinger, T

The photosystem II-phycobilisome preparation, isolated by lauryldimethyl amine oxide treatment, had a greatly reduced chlorophyll content, with an average ratio of 90 chlorophyll a/phycobilisome as compared to approximately 1200 Chl/phycobilisome in unfractionated thylakoids. P700 was not detected in the particles. By electron microsopy the preparations were relatively homogeneous and were generally devoid of chloroplast membranes. In negatively stained preparations phycobilisome particles were seen often in clusters of two and three, probably due to retention of hydrophobic thylakoid fragments. The preparation was deficient in photosystem I chlorophyll complexes, but enriched in polypeptides of 85 to 92, approx. 43, and approx. 26 kDa, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The 43- and 26-kDa polypeptides are attributable to the PS II core and the oxygen-evolving complex, respectively. 27 references, 3 figures, 2 tables.

• Research Organization: Smithosonian Environmental Research Center, Rockville, MD
• DOE Contract Number: AS05-76ER04310
• OSTI ID: 5882462
• Journal Information: Arch. Biochem. Biophys.; (United States), Journal Name: Arch. Biochem. Biophys.; (United States) Vol. 238:1; ISSN ABBIA
• Country of Publication: United States
• Language: English

Related Subjects

14 SOLAR ENERGY
140505 -- Solar Energy Conversion-- Photochemical
Photobiological
& Thermochemical Conversion-- (1980-)
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALGAE
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
CHLOROPHYLL
CHLOROPLASTS
ELECTRON MICROSCOPY
ELECTROPHORESIS
ELEMENTS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
MEMBRANES
MICROSCOPY
NONMETALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN
PEPTIDES
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PHOTOSYNTHETIC REACTION CENTERS
PHYCOBILISOMES
PHYTOCHROMES
PIGMENTS
PLANTS
POLYPEPTIDES
PORPHYRINS
PROTEINS
SAMPLE PREPARATION
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS