Solid-phase radioimmunoassay for human neutrophil elastase: a sensitive method for determining secreted and cell-associated enzyme

Journal Article · Tue Oct 01 04:00:00 EDT 1985 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(85)90436-1· OSTI ID:5880713

Dunn, T L; Blackburn, W D; Koopman, W J; Heck, L W

A solid-phase radioimmunoassay for measuring neutrophil elastase in the range 0.08-4 ng/ml has been developed. A monospecific, precipitating antibody capable of inhibiting elastinolysis was produced by repeated immunizations of a goat. The IgG fraction and affinity-purified antibodies of this serum were then obtained and used to develop this radioimmunoassay. There was no cross-reactivity in binding of the radiolabeled antisera with lactoferrin, cathepsin G, or serine proteinases with amino-terminal amino acid sequence homology. Although serum influences the measurement of catalytically active neutrophil elastase when compared to diisopropylfluorophosphate-treated neutrophil elastase, antigenic elastase may still be measured in body fluids. Furthermore, this assay is more sensitive than commercially available substrates used for quantitating neutrophil elastase by functional activity. We have found this quantitative assay extremely useful in balance studies to measure secreted and cell-associated elastase and in screening of biological fluids for the presence of the enzyme.

• Research Organization: Univ. of Alabama, Birmingham
• OSTI ID: 5880713
• Journal Information: Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 1; ISSN ANBCA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODY FORMATION
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
DOMESTIC ANIMALS
ENZYMES
GOATS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LEUKOCYTES
MAMMALS
MATERIALS
NEUTROPHILS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RUMINANTS
TRACER TECHNIQUES
VERTEBRATES
ZYMOSAN