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Submitochondrial distribution of cAMP in its incubation with rat liver mitochondria

Journal Article · · Biochemistry (Engl. Transl.); (United States)
OSTI ID:5878768
;

When labeled cAMP is incubated with isolated mitochondria from rat liver, it penetrates not only through the outer membrane of the mitochondria but also into the mitoplasts, where it is accumulated chiefly in the matrix. Damage to the mitochondrial membrane by freezing-thawing promotes not an influx but an efflux of cAMP out of the mitoplasts. A substantial part of the labeled nucleotide in all the investigated submitochondrial fractions was identified as intact cAMP by the method of thin-layer chromatography. The transport of cAMP into the mitochondria can explain the activation of their functions by the hormones that activate cytoplasmic adenylate cyclase and by extramitochondrial (cytosol) cAMP, as well as the presence of cAMP-dependent protein kinase and cAMP-phosphodiesterase in the mitochondria.

Research Organization:
Krasnoyarsk State Medical Institute, USSR
OSTI ID:
5878768
Journal Information:
Biochemistry (Engl. Transl.); (United States), Journal Name: Biochemistry (Engl. Transl.); (United States) Vol. 50:9; ISSN BIORA
Country of Publication:
United States
Language:
English

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Related Subjects

550301* -- Cytology-- Tracer Techniques
550501 -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENINES
AMINES
AMP
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
AROMATICS
AZAARENES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BODY
CELL CONSTITUENTS
CHEMILUMINESCENCE
CHEMISTRY
CHROMATOGRAPHY
CYCLASES
DIGESTIVE SYSTEM
DRUGS
ENZYMES
ESTERASES
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROLASES
INCUBATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
LIVER
LIVER CELLS
LUMINESCENCE
LYASES
MAMMALS
METABOLIC ACTIVATION
METABOLISM
MITOCHONDRIA
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
ORGANS
PHOSPHODIESTERASES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PURINES
RADIONUCLIDE KINETICS
RATS
RETENTION
RODENTS
SEPARATION PROCESSES
SOMATIC CELLS
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES