Estimation of guinea pig tracheobronchial transport rates using a compartmental model

Journal Article · Sun Jan 01 04:00:00 EST 1984 · Exp. Lung Res.; (United States)

DOI:https://doi.org/10.3109/01902148409087910· OSTI ID:5878436

Velasquez, D J; Morrow, P E

Mucociliary clearance in the tracheobronchial tree of guinea pigs was examined using monodisperse 7.9 ..mu..m MMAD polystyrene particles. Animals were exposed for approximately 1 h by inhalation via an intratracheal tube to aerosols tagged with gold-198 and fluorescent dyes. Following exposure, animals were radioactively monitored and sacrificed at predetermined times. The lungs were removed, freeze-dried, sectioned completely, and examined with a fluorescent microscope. Measurements were made of airway diameters where particles were found. An anatomic model for guinea pig lung morphology was used to assign ranges of airway diameters to five zones, which were incorporated into a compartmental model for lung clearance. Kinetic analysis of particle distributions in the zones led to development of first-order equations describing the compartmental clearance. Rate constants obtained from the kinetic analysis were used to estimate mucociliary transport rates in specific bronchial generations, which ranged from approximately 0.001 mm/min in the distal bronchioles to approximately 8 mm/min in the trachea, and resulted in a calculated 24-h clearance time for tracheobronchial clearance in the guinea pig. No evidence for either bronchial penetration by particles or relatively prolonged bronchial retention of particles was found in this study. 22 references, 3 figures, 3 tables.

• Research Organization: Univ. of Rochester, NY
• DOE Contract Number: AC02-76EV03490
• OSTI ID: 5878436
• Journal Information: Exp. Lung Res.; (United States), Journal Name: Exp. Lung Res.; (United States) Vol. 7; ISSN EXLRD
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CLEARANCE
DATA
DAYS LIVING RADIOISOTOPES
EQUATIONS
EXCRETION
EXPERIMENTAL DATA
FLUORESCENCE
GOLD 198
GOLD ISOTOPES
GUINEA PIGS
HEAVY NUCLEI
INFORMATION
INHALATION
INTAKE
ISOTOPE APPLICATIONS
ISOTOPES
LUMINESCENCE
LUNG CLEARANCE
LUNGS
MAMMALS
MATERIALS
MATHEMATICAL MODELS
NUCLEI
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC POLYMERS
ORGANS
PARTICLE SIZE
PARTICLES
PARTICULATES
PETROCHEMICALS
PETROLEUM PRODUCTS
PLASTICS
POLYMERS
POLYOLEFINS
POLYSTYRENE
POLYVINYLS
RADIOISOTOPES
RESPIRATORY SYSTEM
RETENTION
RODENTS
SIZE
SYNTHETIC MATERIALS
TRACER TECHNIQUES
TRACHEA
VERTEBRATES