Number and size of human X chromosome fragments transferred to mouse cells by chromosome-mediated gene transfer

Journal Article · Fri May 01 04:00:00 EDT 1981 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.1.5.439· OSTI ID:5877510

Olsen, A S; McBride, O W; Moore, D E

Labeled probes of unique-sequence human X chromosomal deoxyribonucleic acid, prepared by two different procedures, were used to measure the amount of human X chromosomal deoxyribonucleic acid in 12 mouse cell lines expressing human hypoxanthine phosphoribosyltransferase after chromosome-mediated gene transfer. The amount of X chromosomal deoxyribonucleic acid detected by this procedure ranged from undetectable levels in the three stable transformants and some unstable transformants examined to about 20% of the human X chromosome in two unstable transformants. Reassociation kinetics of the X chromosomal probe with deoxyribonucleic acid from the two unstable transformants containing 15 to 20% of the human X chromosome indicate that a single copy of these sequences is present. In one of these lines, the X chromosomal sequences exist as multiple fragments which were not concordantly segregated when the cells were selected for loss of hprt.

• Research Organization: Dept. of Pharmacology, College of Medicine and Dentistry of New Jersey, Rutgers Medical School, Piscataway, NJ 08854
• OSTI ID: 5877510
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 1:5; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
CELL CULTURES
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
CROSSING-OVER
DNA
DNA SEQUENCING
ENZYME ACTIVITY
ENZYMES
GENE OPERONS
GENETIC MAPPING
GLYCOSYL TRANSFERASES
HETEROCHROMOSOMES
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
MAMMALS
MAN
MAPPING
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PENTOSYL TRANSFERASES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PRIMATES
RATS
RODENTS
SIZE
STRUCTURAL CHEMICAL ANALYSIS
TECHNOLOGY ASSESSMENT
TRANSCRIPTION
TRANSFERASES
VERTEBRATES
X CHROMOSOME