Introduction of new genetic markers on human chromosomes

Journal Article · Fri Mar 01 04:00:00 EST 1991 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(91)90531-X· OSTI ID:5268838

Satoh, Hitoshi; Barrett, J C ^[1]; Oshimura, Mitsuo ^[2]

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1. National Inst. of Environmental Health Sciences, Research Triangle Park, NC (United States)
2. Kanagawa Cancer Center Research Inst., Yokohama (Japan)

The purpose of this study was to use DNA transfection and microcell chromosome transfer techniques to engineer a human chromosome containing multiple biochemical markers for which selectable growth conditions exist. The starting chromosome was a t(X;3)(3pter{yields}3p12::Xq26{yields}Xpter) chromosome from a reciprocal translocation in the normal human fibroblast cell line GM0439. This chromosome was transferred to a HPRT (hypoxanthine phosphoribosyltransferase)-deficient mouse A9 cell line by microcell fusion and selected under growth conditions for the HPRT gene on the human t(X;3) chromosome. A resultant HAT-resistant cell line (A9(GM0439)-1) contained a single human t(X;3) chromosome. These results demonstrate that microcell chromosome transfer can be used to select chromosomes containing multiple markers.

• OSTI ID: 5268838
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 193:1; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOLOGICAL MARKERS
CELL CONSTITUENTS
CHROMOSOMES
ENZYMES
GENE RECOMBINATION
GLYCOSYL TRANSFERASES
HETEROCHROMOSOMES
HUMAN CHROMOSOMES
HUMAN X CHROMOSOME
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
ORGANIC COMPOUNDS
PENTOSYL TRANSFERASES
PLASMIDS
PROTEINS
TRANSFERASES
TUMOR CELLS
X CHROMOSOME