Dual pathway for gluconeogenesis from D-glycerate and L-glycerate

Conference · Fri May 01 00:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5877259

Chen, K S; Lardy, H A; Katz, J

In freshly isolated hepatocytes, the time course and substrate concentration dependences of glucose synthesis from D-glycerate are similar to those from L-glycerate even though the cellular content of D-glycerate was markedly less when L-glycerate was the substrate. Glucose synthesis from the isomers were differentially affected by various metabolic inhibitors. These differences cannot be fully explained by the relatively more sensitive nature of gluconeogenesis from L-glycerate to cytosolic NADH/NAD ratio. The results indicate that only a portion of the glucose formed from D- and L-glycerate involves the well-known pathway comprising lactate dehydrogenase, D-glycerate dehydrogenase and D-glycerate kinase for generation of 2-phospho-D-glycerate from D- and L-glycerate. Studies employing /sup 14/C formaldehyde indicate an additional pathway involving transketolase for gluconeogenesis from these substrates.

• Research Organization: Univ. of Madison, WI
• OSTI ID: 5877259
• Report Number(s): CONF-870644-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 46:6; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMAL CELLS
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBOHYDRATES
CARBON 14 COMPOUNDS
COENZYMES
ENZYMES
FORMALDEHYDE
GLUCOSE
HEMIACETAL DEHYDROGENASES
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LACTATE DEHYDROGENASE
LIVER CELLS
MONOSACCHARIDES
NAD
NADH2
NUCLEOTIDES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
SACCHARIDES
SOMATIC CELLS
SYNTHESIS
TRACER TECHNIQUES
TRANSFERASES