Cross-linking of surface Ig receptors on murine B lymphocytes stimulates the expression of nuclear tetradecanoyl phorbol acetate-response element-binding proteins

Journal Article · Thu Mar 14 23:00:00 EST 1991 · Journal of Immunology; (USA)

OSTI ID:5868125

Chiles, T C; Liu, J L; Rothstein, T L ^[1]

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1. Boston Univ. Medical Center, MA (USA)

Cross-linking of sIg on primary B lymphocytes leads to increased nuclear DNA-binding activity specific for the tetradecanoyl phorbol acetate-response element (TRE), as judged by gel mobility shift assays. Stimulation of B cells to enter S phase of the cell cycle by treatment with the combination of phorbol ester plus calcium ionophore also stimulated nuclear TRE-binding activity within 2 h, with maximal expression at 4 h; however, phorbol ester and calcium ionophore were not as effective in stimulating binding activity when examined separately. Stimulated nuclear expression of TRE-binding activity appears to require protein synthesis. Fos- and Jun/AP-1-related proteins participate directly in the identified nucleoprotein complex, as shown by the ability of c-fos- and c-jun-specific antisera to either alter or completely abolish electrophoretic migration of the complex in native gels. Further, UV photo-cross-linking studies identified two major TRE-binding protein species, whose sizes correspond to TRE-binding proteins derived from HeLa cell nuclear extracts. The results suggest that in primary B cells nuclear TRE-binding activity represents a downstream signaling event that occurs subsequent to changes in protein kinase C activity and intracellular Ca2+ but that can be triggered physiologically through sIg.

• OSTI ID: 5868125
• Journal Information: Journal of Immunology; (USA), Journal Name: Journal of Immunology; (USA) Vol. 146:6; ISSN JOIMA; ISSN 0022-1767
• Country of Publication: United States
• Language: English

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560120 -- Radiation Effects on Biochemicals
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CELL CONSTITUENTS
CELL CYCLE
CELL NUCLEI
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
CROSS-LINKING
ELECTROMAGNETIC RADIATION
ESTERS
GENE REGULATION
GLOBULINS
IMMUNOGLOBULINS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MICE
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PHORBOL ESTERS
POLYMERIZATION
PROTEINS
RADIATIONS
RECEPTORS
RODENTS
SOMATIC CELLS
TRANSCRIPTION FACTORS
ULTRAVIOLET RADIATION
VERTEBRATES