Cross-linking of surface Ig receptors on murine B lymphocytes stimulates the expression of nuclear tetradecanoyl phorbol acetate-response element-binding proteins
- Boston Univ. Medical Center, MA (USA)
Cross-linking of sIg on primary B lymphocytes leads to increased nuclear DNA-binding activity specific for the tetradecanoyl phorbol acetate-response element (TRE), as judged by gel mobility shift assays. Stimulation of B cells to enter S phase of the cell cycle by treatment with the combination of phorbol ester plus calcium ionophore also stimulated nuclear TRE-binding activity within 2 h, with maximal expression at 4 h; however, phorbol ester and calcium ionophore were not as effective in stimulating binding activity when examined separately. Stimulated nuclear expression of TRE-binding activity appears to require protein synthesis. Fos- and Jun/AP-1-related proteins participate directly in the identified nucleoprotein complex, as shown by the ability of c-fos- and c-jun-specific antisera to either alter or completely abolish electrophoretic migration of the complex in native gels. Further, UV photo-cross-linking studies identified two major TRE-binding protein species, whose sizes correspond to TRE-binding proteins derived from HeLa cell nuclear extracts. The results suggest that in primary B cells nuclear TRE-binding activity represents a downstream signaling event that occurs subsequent to changes in protein kinase C activity and intracellular Ca2+ but that can be triggered physiologically through sIg.
- OSTI ID:
- 5868125
- Journal Information:
- Journal of Immunology; (USA), Vol. 146:6; ISSN 0022-1767
- Country of Publication:
- United States
- Language:
- English
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IMMUNOGLOBULINS
RECEPTORS
NUCLEOPROTEINS
GENE REGULATION
PHORBOL ESTERS
BIOLOGICAL EFFECTS
CELL CYCLE
CELL NUCLEI
CROSS-LINKING
LYMPHOCYTES
MICE
TRANSCRIPTION FACTORS
ULTRAVIOLET RADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
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BODY FLUIDS
CARCINOGENS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
ESTERS
GLOBULINS
LEUKOCYTES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
POLYMERIZATION
PROTEINS
RADIATIONS
RODENTS
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
560120 - Radiation Effects on Biochemicals
Cells
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