Molecular characterization of nosA, a Pseudomonas stutzeri gene encoding an outer membrane protein required to make copper-containing N sub 2 O reductase
- Univ. of California, Davis (United States)
- Georgia State Univ., Atlanta (United States)
A Pseudomonas stutzeri gene (nosA) encoding an outer membrane protein was cloned into the broad-host-range vector pRK290 and expressed in a mutant lacking the protein. Deletion analysis identified the approximate extent of the nosA region which was sequenced, and it was found to contain an open reading frame encoding 683 amino acids including a presumed signal sequence of 44 amino acids. The putative processed form had a molecular weight of 80,218, characteristic typical of outer membrane proteins, and considerable amino acid sequence homology with Escherichia coli BtuB. A short stretch of amino acids homologous with the E. coli TonB-dependent outer membrane proteins, BtuB, IutA, FepA, and FhuA, suggesting a homologous function: interaction with a periplasmic protein or uptake of a specific substrate.
- DOE Contract Number:
- FG03-85ER13356
- OSTI ID:
- 5865628
- Journal Information:
- Journal of Bacteriology; (United States), Vol. 173:17; ISSN 0021-9193
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
OXIDOREDUCTASES
GENES
PSEUDOMONAS
MOLECULAR BIOLOGY
AMINO ACID SEQUENCE
COPPER
DNA SEQUENCING
DNA-CLONING
ESCHERICHIA COLI
MUTANTS
NITROUS OXIDE
BACTERIA
CHALCOGENIDES
CLONING
DNA HYBRIDIZATION
ELEMENTS
ENZYMES
HYBRIDIZATION
METALS
MICROORGANISMS
MOLECULAR STRUCTURE
NITROGEN COMPOUNDS
NITROGEN OXIDES
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
TRANSITION ELEMENTS
550200* - Biochemistry