Molecular characterization of nosA, a Pseudomonas stutzeri gene encoding an outer membrane protein required to make copper-containing N sub 2 O reductase

Journal Article · Sun Sep 01 00:00:00 EDT 1991 · Journal of Bacteriology; (United States)

OSTI ID:5865628

Lee, H S; Clark, M A; Ingraham, J L ^[1]; Abdelal, A H.T. ^[2]

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1. Univ. of California, Davis (United States)
2. Georgia State Univ., Atlanta (United States)

A Pseudomonas stutzeri gene (nosA) encoding an outer membrane protein was cloned into the broad-host-range vector pRK290 and expressed in a mutant lacking the protein. Deletion analysis identified the approximate extent of the nosA region which was sequenced, and it was found to contain an open reading frame encoding 683 amino acids including a presumed signal sequence of 44 amino acids. The putative processed form had a molecular weight of 80,218, characteristic typical of outer membrane proteins, and considerable amino acid sequence homology with Escherichia coli BtuB. A short stretch of amino acids homologous with the E. coli TonB-dependent outer membrane proteins, BtuB, IutA, FepA, and FhuA, suggesting a homologous function: interaction with a periplasmic protein or uptake of a specific substrate.

• DOE Contract Number: FG03-85ER13356
• OSTI ID: 5865628
• Journal Information: Journal of Bacteriology; (United States), Journal Name: Journal of Bacteriology; (United States) Vol. 173:17; ISSN JOBAA; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
BACTERIA
CHALCOGENIDES
CLONING
COPPER
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
ELEMENTS
ENZYMES
ESCHERICHIA COLI
GENES
HYBRIDIZATION
METALS
MICROORGANISMS
MOLECULAR BIOLOGY
MOLECULAR STRUCTURE
MUTANTS
NITROGEN COMPOUNDS
NITROGEN OXIDES
NITROUS OXIDE
ORGANIC COMPOUNDS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PROTEINS
PSEUDOMONAS
STRUCTURAL CHEMICAL ANALYSIS
TRANSITION ELEMENTS