Age-related changes in receptor-mediated phosphoinositide hydrolysis in various regions of rat brain

Journal Article · Mon Dec 31 23:00:00 EST 1990 · Life Sciences; (United States)

DOI:https://doi.org/10.1016/0024-3205(91)90308-X· OSTI ID:5865382

Mundy, W; Tandon, P; Tilson, H ^[1]; Ali, S ^[2]

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1. Research Triangle Park, NC (United States)
2. National Center for Toxicological Research, Jefferson, AR (United States)

The effects of age on cholinergic markers and receptor-stimulated phosphoinositide hydrolysis was examined in the frontal cortex and striatum of male Fischer-344 rats. Choline acetyltransferase activity was decreased 27% in the striatum of aged rats compared to young controls. Muscarinic receptor density as measured by ({sup 3}H)-quinuclidinyl benzilate binding showed a similar 26% decrease in the striatum of aged rats. Phosphoinositide hydrolysis was measured by the release of inositol phosphate (IP) from tissue slices prelabeled with ({sup 3}H)myoinositol in response to carbachol, norepinephrine, and quisqualate. In the cortex, stimulated IP release was significantly greater in slices from aged rats compared to young rats for all three agonists. In contrast, stimulated IP release was significantly decreased in striatal slices from aged rats compared to young for all three agonists. These data indicate a differential effect of age on agonist-stimulated phosphoinositide hydrolysis in the cortex and striatum. The decreased responsiveness in the latter area may result from the age-related loss of postsynaptic receptors.

• OSTI ID: 5865382
• Journal Information: Life Sciences; (United States), Journal Name: Life Sciences; (United States) Vol. 49:14; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGE DEPENDENCE
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CARBOHYDRATES
CENTRAL NERVOUS SYSTEM
CHOLINE
DRUGS
ENZYME ACTIVITY
ENZYMES
ESTERS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INOSITOL
INOSITOLS
ISOTOPE APPLICATIONS
KINETICS
LIPIDS
LIPOTROPIC FACTORS
MEMBRANE PROTEINS
METABOLISM
MONOSACCHARIDES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
PROTEINS
QUATERNARY COMPOUNDS
REACTION KINETICS
RECEPTORS
SACCHARIDES
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS