Short chain fatty acids and cadmium cytotoxicity in ROS 17/2. 8 cells

Conference · Mon Mar 11 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5863410

Thomas, D J; Angle, C R; Swanson, S A ^[1]

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1. Univ. of Nebraska, Omaha (United States)

ROS 17/2.8 rat osteosarcoma cells are extremely sensitive to the cytotoxic effects of Cd. In naive cells, sensitivity to Cd is associated with poor inducibility of metallothionein (MT). Treatment of ROS 17/2.8 cells with Na butyrate (NaB) results in increased resistance to Cd cytotoxicity and increased MT gene expression. The relation between the structure of short chain fatty acids and the alterations of Cd cytotoxicity in ROS 17/2.8 cells was investigated by culture of cells in the presence of 1 or 5 mM Na acetate, NaB, methyl butyrate, isobutyric acid, methyl isobutyrate and caproic acid. Among these compounds, only 5 mM NaB significantly increased the survival of ROS cells exposed to 0.1 to 10 {mu}M Cd and only NaB treatment was effective in increasing MT gene inducibility. The role of inhibition of DNA replication by NaB in cell resistance to Cd was examined. Among compounds tested, only 5 mM NaB significantly inhibited DNA synthesis. However, in cells in which DNA synthesis is inhibited by exposure to hydroxyurea, addition and removal of NaB from culture medium modulates cellular resistance to Cd. Hence, the effectiveness of NaB as a modifier of cell response to Cd is not due entirely to changes in cell proliferation. Additionally, rigid structural constraints for effectiveness dictate that only NaB is a potent modifier of resistance to Cd.

• OSTI ID: 5863410
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:4
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL COMPOUNDS
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BUTYRIC ACID
CADMIUM
CARBOXYLIC ACIDS
CELL PROLIFERATION
DISEASES
DNA REPLICATION
ELEMENTS
GENE REGULATION
KINETICS
MAMMALS
METALLOPROTEINS
METALLOTHIONEIN
METALS
MOLECULAR STRUCTURE
MONOCARBOXYLIC ACIDS
NEOPLASMS
NUCLEIC ACID REPLICATION
ORGANIC ACIDS
ORGANIC COMPOUNDS
OSTEOSARCOMAS
PROTEINS
RATS
REACTION KINETICS
RODENTS
SARCOMAS
SKELETAL DISEASES
SODIUM COMPOUNDS
TOXICITY
TUMOR CELLS
VERTEBRATES