Rapid agonist-induced loss of sup 125 I-. beta. -endorphin opioid receptor sites in NG108-15, but not SK-N-SH neuroblastoma cells

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Life Sciences; (United States)

OSTI ID:5863136

Cone, R I; Lameh, J; Sadee, W ^[1]

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1. Univ. of California, San Francisco (United States)

The authors have measured {mu} and {delta} opioid receptor sites on intact SK-N-SH and NG108-15 neuroblastoma cells, respectively, in culture. Use of {sup 125}I-{beta}-endorphin ({beta}E) as a tracer, together with {beta}E(6-31) to block high-affinity non-opioid binding in both cell lines, permitted the measurement of cell surface {mu} and {delta} opioid receptor sites. Labeling was at {delta} sites in NG108-15 cells and predominantly at {mu} sites in SK-N-SH cells. Pretreatment with the {mu} and {delta} agonist, DADLE, caused a rapid loss of cell surface {delta} receptor sites in NG108-15 cells, but failed to reduce significantly {mu} receptor density in SK-N-SH cells.

• OSTI ID: 5863136
• Journal Information: Life Sciences; (United States), Vol. 49:19; ISSN 0024-3205
• Country of Publication: United States
• Language: English

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