Opioid receptors in human neuroblastoma SH-SY5Y cells: evidence for distinct morphine (. mu. ) and enkephalin (delta) binding sites

Journal Article · Fri Jun 13 00:00:00 EDT 1986 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(86)91152-6· OSTI ID:7245141

Kazmi, S M.I.; Mishra, R K

Human neuroblastoma SH-SY5Y cells exhibited a heterogeneous population of ..mu.. and delta types of opioid binding sites. These specific binding sites displayed the characteristic saturability, stereospecificity and reversibility, expected of a receptor. Scatchard analysis of (/sup 3/H)-D-Ala/sup 2/-D-Leu/sup 5/-enkephalin (DADLE) in the presence of 10/sup -5/M D-Pro/sup 4/-morphiceptin (to block the ..mu.. receptors) and the competitive displacement by various highly selective ligands yielded the binding parameters of delta sites which closely resemble those of the delta receptors in brain and mouse neuroblastoma clones. Similarly, the high affinity binding of (/sup 3/H)-dihydromorphine, together with the higher potency of morphine analogues to displace (/sup 3/H)-naloxone binding established the presence of ..mu.. sites. Guanine nucleotides and NaCl significantly inhibited the association and increased the dissociation of (/sup 3/H)-DADLE binding.

• Research Organization: McMaster Univ., Hamilton, Ontario
• OSTI ID: 7245141
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 137:2; ISSN BBRCA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL COMPOUNDS
ALKALOIDS
AMINES
ANALGESICS
ANIMAL CELLS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
BIOCHEMICAL REACTION KINETICS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHLORIDES
CHLORINE COMPOUNDS
DATA
DRUGS
ENDORPHINS
ENKEPHALINS
EXPERIMENTAL DATA
GUANINE
HALIDES
HALOGEN COMPOUNDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INFORMATION
INHIBITION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MEMBRANE PROTEINS
MORPHINE
NARCOTICS
NERVE CELLS
NEUROREGULATORS
NUCLEOTIDES
NUMERICAL DATA
OPIUM
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
PURINES
REACTION KINETICS
RECEPTORS
SODIUM CHLORIDES
SODIUM COMPOUNDS
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS