Glutamate decarboxylase immunoreactivity and gamma-(/sup 3/H) aminobutyric acid accumulation within the same neurons in dissociated cell cultures of cerebral cortex

Journal Article · Tue Feb 01 04:00:00 EST 1983 · J. Neurosci.; (United States)

OSTI ID:5861392

Neale, E A; Oertel, W H; Bowers, L M; Weise, V K

In order to evaluate the reliability of high affinity (/sup 3/H)GABA accumulation as a marker for GABAergic neurons, murine cerebral cortical neurons were studied in dissociated cell culture. Cultures which had been incubated in (/sup 3/H)GABA were stained immunohistochemically for the GABA-synthesizing enzyme, glutamate decarboxylase, fixed with paraformaldehyde, and subsequently processed for radioautography. In mature cultures, there was an 84 to 94% correlation between the presence of the enzyme and (/sup 3/H)GABA uptake within the same cortical neurons. These data provide direct evidence that those neurons which synthesize GABA are the same neurons which are labeled by high affinity (/sup 3/H)GABA uptake.

• Research Organization: National Inst. of Child Health and Human Development, Bethesda, MD
• OSTI ID: 5861392
• Journal Information: J. Neurosci.; (United States), Journal Name: J. Neurosci.; (United States) Vol. 3:2; ISSN JNRSD
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AMINOBUTYRIC ACID
ANIMAL CELLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
BIOCHEMISTRY
BIOLOGICAL LOCALIZATION
BODY
BRAIN
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
CEREBRAL CORTEX
CEREBRUM
CHEMISTRY
CYTOCHEMISTRY
DECARBOXYLASES
DRUGS
ENZYMES
GLUTAMIC ACID
IN VITRO
LABELLED COMPOUNDS
LYASES
MAMMALS
MEMBRANE TRANSPORT
MICE
NERVE CELLS
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RODENTS
SOMATIC CELLS
TRITIUM COMPOUNDS
VERTEBRATES