Localization of glycine, GABA and neuropeptide containing neurons in tiger salamander retina

Thesis/Dissertation · Fri Jan 01 04:00:00 EST 1988

OSTI ID:5162021

Yang, C Y

Putative glycinergic and GABAergic neurons in the salamander retina were localized by a parallel analysis of high affinity {sup 3}H-glycine uptake and glycine-like immunoreactivity (Gly-IR) and a comparative analysis of high affinity {sup 3}H-GABA uptake, GABA, like immunoreactivity (GABA-IR), and glutamate decarboxylase immunoreactivity (GAD-IR) at the light microscopic level. Good correspondence of labeling of {sup 3}H-glycine uptake and Gly-IR as well as that of {sup 3}H-GABA uptake and GABA-IR were observed. In addition, GAD immunoreactive neurons contained GABA-IR as well. Extensive colocalization of {sup 3}H-glycine uptake and Gly-IR and that of {sup 3}H-GABA uptake, GABA-IR and perhaps GAD-IR were indicated by the similarities in the distribution, morphology and labeling frequency of neurons and lamination in the inner plexiform layer (IPL). However, the Gly-IR and the GABA-IR probes appeared to be more sensitive and can thus be a reliable marker for glycine and GABA containing neurons respectively.

• Research Organization: State Univ. of New York, Stony Brook, NY (USA)
• OSTI ID: 5162021
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AMINOBUTYRIC ACID
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL LOCALIZATION
BIOLOGICAL MARKERS
BODY
BODY AREAS
CARBOXYLIC ACIDS
DRUGS
EYES
FACE
GLYCINE
HEAD
HYDROGEN COMPOUNDS
IMMUNE REACTIONS
ISOTOPE APPLICATIONS
NERVE CELLS
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RETINA
SALAMANDERS
SENSE ORGANS
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES