Bioreactor for the study of defined interactions of toxic metals and biofilms
Journal Article
·
· Appl. Environ. Microbiol.; (United States)
OSTI ID:5858726
A novel bioreactor system constructed for studies of the interactions of heavy metals and microbial cells at the solid-solution interface is described. The applicability of this experimental system to meet the severe constraints imposed on such an apparatus by the requirements for an unambiguous interpretation of data and for mathematical modeling of these interactions was explored with the trace metal lead and with the marine bacterium Pseudomonas atlantica. A chemically defined medium composed of the major components of seawater, simple salts required for growth, glucose, and the single amino acid glycine was derived. It supported a maximum growth rate several times less than that in a complex medium, but provided growth to high cell densities and the formation of biopolymer and supported the development of a monolayer biofilm. The use of such a medium in conjunction with our bioreactor system minimized trace metal contamination while allowing quantification of the partitioning of lead onto various reactor surfaces. Lead adsorption by reactor walls and model surfaces was linear with equilibrium led concentration up to 6 X 10(-6) mol/liter. Equilibrium lead adsorption due to P. atlantica biofilm surfaces ranged from 20 to 40% at a total lead concentration of 10(-6) mol/liter depending upon solution pH and ionic composition, indicating that biofilms can play an important role in controlling toxic metal concentrations in natural systems.
- Research Organization:
- Cornell Univ., Ithaca, NY
- OSTI ID:
- 5858726
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 5; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
Similar Records
Interactions of microbial biofilms with toxic trace metals; 2: Prediction and verification of an integrated computer model of lead (II) distribution in the presence of microbial activity
Interactions of microbial biofilms with toxic trace metals; 1: Observation and modeling of cell growth, attachment, and production of extracellular polymer
Modeling interactions of toxic metals with suspended bacterial cells, extracellular polymer and biofilms in a simulated freshwater environment
Journal Article
·
Mon Jun 20 00:00:00 EDT 1994
· Biotechnology and Bioengineering; (United States)
·
OSTI ID:7016283
Interactions of microbial biofilms with toxic trace metals; 1: Observation and modeling of cell growth, attachment, and production of extracellular polymer
Journal Article
·
Mon Jun 20 00:00:00 EDT 1994
· Biotechnology and Bioengineering; (United States)
·
OSTI ID:7272553
Modeling interactions of toxic metals with suspended bacterial cells, extracellular polymer and biofilms in a simulated freshwater environment
Conference
·
Thu Nov 30 23:00:00 EST 1995
·
OSTI ID:126546
Related Subjects
560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOREACTORS
CULTURE MEDIA
DESIGN
ELEMENTS
FILMS
GROWTH
LEAD
METALS
MICROORGANISMS
POLYMERS
PSEUDOMONAS
TRACE AMOUNTS
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOREACTORS
CULTURE MEDIA
DESIGN
ELEMENTS
FILMS
GROWTH
LEAD
METALS
MICROORGANISMS
POLYMERS
PSEUDOMONAS
TRACE AMOUNTS