Further identification of endogenous gibberellins in the shoots of pea, line G2

Journal Article · Fri Dec 01 00:00:00 EST 1989 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.91.4.1255· OSTI ID:5858254

Halinska, A; Davies, P J; Lee, J W; Zhu, Yuxian ^[1]

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1. Cornell Univ., Ithaca, NY (USA)

To interpret the metabolism of radiolabeled gibberellins A{sub 12}-aldehyde and A{sub 12} in shoots of pea (Pisum sativum L.), the identity of the radiolabeled peaks has to be determined and the endogenous presence of the gibberellins demonstrated. High specific activity ({sup 14}C)GA{sub 12} and ({sup 14}C)GA{sub 12}-aldehyde were synthesized using a pumpkin endosperm enzyme preparation, and purified by high performance liquid chromatography (HPLC). ({sup 14}C)GA{sub 12} was supplied to upper shoots of pea, line G2, to produce radiolabeled metabolites on the 13-OH pathway. Endogenous compounds copurifying with the ({sup 14}C)GAs on HPLC were analyzed by gas chromatography-mass spectrometry. The endogenous presence of GA{sub 53}, GA{sub 44}, GA{sub 19} and GA{sub 20} was demonstrated and their HPLC peak identity ascertained. The {sup 14}C was progressively diluted in GAs further down the pathway, proportional to the levels found in the tissue and inversely proportional to the speed of metabolism, ranging from 63% in GA{sub 53} to 4% in GA{sub 20}. Calculated levels of GA{sub 20}, GA{sub 19}, GA{sub 44}, and GA{sub 53} were 42, 8, 10, and 0.5 nanograms/gram, respectively.

• OSTI ID: 5858254
• Journal Information: Plant Physiology; (USA), Vol. 91:4; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GIBBERELLIC ACID
BIOLOGICAL PATHWAYS
CARBON 14 COMPOUNDS
METABOLISM
PISUM
TRACER TECHNIQUES
CARBOXYLIC ACIDS
ESTERS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LACTONES
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
550201* - Biochemistry- Tracer Techniques