Biosynthesis of vitamin B12: concerning the origin of the methine protons of the corrin nucleus

Journal Article · Thu Oct 01 00:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.19.6616· OSTI ID:5856967

Scott, A I; Kajiwara, M; Santander, P J

13C NMR spectroscopy has been used to locate six deuterium atoms incorporated biosynthetically on the periphery of the corrin nucleus of vitamin B12 (cyanocobalamin) derived from cells of Propionibacterium shermanii grown in a medium containing 50% /sup 2/H/sub 2/O and /sup 13/C-enriched delta-aminolevulinic acid. The implications of these results for the mechanism of vitamin B12 biosynthesis are discussed, and it is concluded that the same oxidation level of the intermediates is maintained throughout the biosynthetic pathway, from delta-aminolevulinic acid to corrin.

• Research Organization: Texas A & M Univ., College Station, TX (United States)
• OSTI ID: 5856967
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:19
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
VITAMIN B-12
BIOSYNTHESIS
MOLECULAR STRUCTURE
CARBON 13
DEUTERIUM
NUCLEAR MAGNETIC RESONANCE
TRACER TECHNIQUES
CARBON ISOTOPES
DRUGS
EVEN-ODD NUCLEI
HEMATINICS
HEMATOLOGIC AGENTS
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
NUCLEI
ODD-ODD NUCLEI
RESONANCE
STABLE ISOTOPES
SYNTHESIS
VITAMIN B GROUP
VITAMINS
550201* - Biochemistry- Tracer Techniques