Biosynthesis of vitamin B12: concerning the origin of the methine protons of the corrin nucleus
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
13C NMR spectroscopy has been used to locate six deuterium atoms incorporated biosynthetically on the periphery of the corrin nucleus of vitamin B12 (cyanocobalamin) derived from cells of Propionibacterium shermanii grown in a medium containing 50% /sup 2/H/sub 2/O and /sup 13/C-enriched delta-aminolevulinic acid. The implications of these results for the mechanism of vitamin B12 biosynthesis are discussed, and it is concluded that the same oxidation level of the intermediates is maintained throughout the biosynthetic pathway, from delta-aminolevulinic acid to corrin.
- Research Organization:
- Texas A and M Univ., College Station
- OSTI ID:
- 5856967
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:19; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOSYNTHESIS
CARBON 13
CARBON ISOTOPES
DEUTERIUM
DRUGS
EVEN-ODD NUCLEI
HEMATINICS
HEMATOLOGIC AGENTS
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MOLECULAR STRUCTURE
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-ODD NUCLEI
RESONANCE
STABLE ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
VITAMIN B GROUP
VITAMIN B-12
VITAMINS
59 BASIC BIOLOGICAL SCIENCES
BIOSYNTHESIS
CARBON 13
CARBON ISOTOPES
DEUTERIUM
DRUGS
EVEN-ODD NUCLEI
HEMATINICS
HEMATOLOGIC AGENTS
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MOLECULAR STRUCTURE
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-ODD NUCLEI
RESONANCE
STABLE ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
VITAMIN B GROUP
VITAMIN B-12
VITAMINS