Changes in the synthesis of DNA, purine nucleotides, and the sensitivity to glucocorticoids in lymphoid cells, coupled with disorders of differentiation and immune function in mice during tumor growth. Thymocytes

Journal Article · Sat Sep 20 04:00:00 EDT 1986 · Biochemistry (Engl. Transl.); (United States)

OSTI ID:5856292

Potapova, G I; Khramtsova, S N; Dmitrieva, L V; Shapot, V S

Certain biochemical mechanisms underlying the impairment of cellular immunity were studies on models of transplanted and orthoaminoazotoluene-induced solid hepatomas in C/sub 3/HA mice. In the thymocytes of the mice, by the beginning of rapid growth of the hepatomas, a significant decrease in the adenosine deaminase was detected, six fold relative to the control, resulting in a corresponding 6-7-fold increase in the intracellular concentration of dATP and dGTP, potential inhibitors of ribonucleoside diphosphate reductase. These disorders were accompanied by inhibition of DNA synthesis, recordable according to the decrease in the thymidine kinase activity, the rate of incorporation of (/sup 14/C)thymidine into the thymocyte DNA in vitro, and the pool of dTTP and dCTP. At the terminal periods of growth of transplanted and induced hepatomas, 3- and 8-fold increases in the corticosterone concentration in the blood serum of the tumor-bearing animals were detected, respectively. An increase in the specific binding of (/sup 3/H)triamsinolone acetonide by the thymocytes and an increase in their terminal deoxynucleotide transferase activity were also established, which, among other indices, reflects a lag in the differentiation of T-lymphocytes at the level of the cortical thymocytes.

• Research Organization: Scientific-Research Institute of Carcinogenesis, Moscow, USSR
• OSTI ID: 5856292
• Journal Information: Biochemistry (Engl. Transl.); (United States), Journal Name: Biochemistry (Engl. Transl.); (United States) Vol. 51:3; ISSN BIORA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANIMAL CELLS
ANIMALS
AROMATICS
ATP
AZAARENES
AZINES
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBON 14 COMPOUNDS
CARCINOGENESIS
CELL DIFFERENTIATION
CHEMISTRY
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
CYTOCHEMISTRY
DISEASES
DNA
ENZYME ACTIVITY
ENZYMES
GLUCOCORTICOIDS
HEPATOMAS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMMUNOSUPPRESSION
KETONES
LABELLED COMPOUNDS
LABELLING
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
METABOLISM
MICE
NEOPLASMS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOGENESIS
PREGNANES
PURINES
PYRIMIDINES
RADIOENZYMATIC ASSAY
RIBOSIDES
RODENTS
SOMATIC CELLS
STEROIDS
SYNTHESIS
THYMIDINE
THYMOCYTES
TRANSFERASES
TRANSPLANTS
TUMOR CELLS
UPTAKE
VERTEBRATES