Rapid changes in deoxynucleoside triphosphate pools in mammalian cells treated with mutagens

Journal Article · Fri Jul 29 04:00:00 EDT 1983 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(83)90802-1· OSTI ID:6250297

Das, S K; Benditt, E P; Loeb, L A

The rapid increase in cellular deoxynucleoside triphosphate (dNTP) concentrations in Chinese Hamster cell line V79 after exposure to known mutagens is described. With this cell line an expansion of dATP and dTTP pools was detected; changes in dCTP were not large; changes in dGTP were either not significant or too low to quantitate. This situation may reflect the existence of imbalances in dNTP pools at the DNA replication fork. The expansion of dATP and dTTP pools occurred within 2 to 4 hours after exposure of cultured cells to N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Ultraviolet light (UV), mitomycin C, and cytosine arabinoside also caused similar dNTP pool changes.

• Research Organization: Univ. of Washington, Seattle
• OSTI ID: 6250297
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 114:2; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
AZINES
BIOCHEMICAL REACTION KINETICS
CARBONIC ACID DERIVATIVES
CHO CELLS
CYTOSINE
DRUGS
ELECTROMAGNETIC RADIATION
GUANIDINES
HETEROCYCLIC COMPOUNDS
KINETICS
MITOMYCIN
MUTAGENESIS
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PYRIMIDINES
RADIATIONS
REACTION KINETICS
ULTRAVIOLET RADIATION