Evidence for an indirect effect of radiation on mammalian chromosomes. III. UV- and x-ray-induced sister chromatid exchanges in heterokaryons

Journal Article · Fri Apr 01 04:00:00 EST 1983 · Cancer Genet. Cytogenet.; (United States)

DOI:https://doi.org/10.1016/0165-4608(83)90073-0· OSTI ID:5853672

Graves, J A; Kellow, G N

The hypothesis that chromosomes may be damaged indirectly by radiation was examined by assaying sister chromatid exchange, (SCE) frequency in heterokaryons between irradiated and unirradiated mouse and Chinese hamster cells. One cell line was UV or x irradiated, then fused to unirradiated BrdU-labeled cells of the other line; SCEs in the unirradiated set were scored in heterokaryons. A dose-dependent increase was consistently observed; the magnitude of which suggested that 25% of UV-induced and up to 60% of x-ray-induced SCEs are indirectly induced. Medium transfer experiments, cell mixing, and fusion with irradiated chick erythrocyte ghosts suggested that unirradiated chromosomes in heterokaryons are damaged by a stable, nondiffusible cytoplasmic component contributed by the irradiated cell.

• Research Organization: Department of Genetics and Human Variation, La Trobe University, Bundoora, Victoria, Australia
• OSTI ID: 5853672
• Journal Information: Cancer Genet. Cytogenet.; (United States), Journal Name: Cancer Genet. Cytogenet.; (United States) Vol. 8:4; ISSN CGCYD
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
ELECTROMAGNETIC RADIATION
HAMSTERS
IONIZING RADIATIONS
MAMMALS
MICE
MUTATIONS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RODENTS
SISTER CHROMATID EXCHANGES
ULTRAVIOLET RADIATION
VERTEBRATES
X RADIATION