Metallothionein in rabbit kidneys preserved for transplantation

Journal Article · Thu Mar 01 04:00:00 EST 1984 · Environ. Health Perspect.; (United States)

DOI:https://doi.org/10.1289/ehp.8454275· OSTI ID:5853137

Elinder, C G; Lundgren, G; Nordberg, M; Palm, B; Piscator, M

Thirteen rabbits were given cadmium injections to achieve cadmium concentrations in kidney cortex ranging from 0.05 to 1 mmole Cd/kg wet weight. Another four animals served as controls. One kidney from each animal was frozen directly to -70/sup 0/C whereas the other kidney was kept for 24 hr at +4/sup 0/C in a preservative (Sachs' solution) to simulate conditions for preservation of human donor kidneys before transplantation. Protein binding of cadmium, zinc and copper in kidney homogenates and the concentration of metallothionein (MT) were measured in the kidney that was frozen directly and in the kidney that had been preserved. No gross differences in either the protein binding of cadmium, zinc and copper or in the MT content were seen between the directly frozen and preserved kidney from the same animal. This indicates that MT is not rapidly broken down in rabbit kidneys which have been preserved similarly to human donor kidneys for 24 hr in a standard preservative solution prior to a transplantation. 27 references, 3 figures, 1 table.

• Research Organization: National Board of Occupational Safety and Health, Solna, Sweden
• OSTI ID: 5853137
• Journal Information: Environ. Health Perspect.; (United States), Journal Name: Environ. Health Perspect.; (United States) Vol. 54; ISSN EVHPA
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOCHEMICAL REACTION KINETICS
BODY
CADMIUM
CHEMICAL BONDS
COMPARATIVE EVALUATIONS
COPPER
DATA
ELEMENTS
EXPERIMENTAL DATA
INFORMATION
INJECTION
INTAKE
KIDNEYS
KINETICS
MAMMALS
METALLOPROTEINS
METALLOTHIONEIN
METALS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
PRESERVATION
PROTEINS
RABBITS
REACTION KINETICS
RETENTION
SAMPLE PREPARATION
SIMULATION
TRANSITION ELEMENTS
TRANSPLANTS
VERTEBRATES
ZINC