Incorporation of cytokinins into DNA of wheat seedlings
Journal Article
·
· Biochemistry (Engl. Transl.); (United States)
OSTI ID:5851573
After incubation of wheat seedlings with (/sup 3/H)benzylaminopurine or (8-/sup 3/H)zeathin appreciable radioactivity was found in the purine bases of DNA, mainly in adenine. Moreover, as a result of acid hydrolysis labeled cytokinins were isolated from labeled highly purified preparations of the total DNA of wheat seedlings. Their radioactivity constituted 5-10% of the radioactivity of the adenine bases in the DNA. After mild acid hydrolysis (/sup 3/H)benzylaminopurine and (/sup 3/H)zeathin were found in the deoxyribonucleoside fraction obtained from cytokinin-labeled DNA. Thus, different cytokinins (N/sup 6/-substituted adenine derivatives) are covalently incorporated into the newly synthesized DNA of plants.
- Research Organization:
- M. V. Lomonosov Moscow State Univ., USSR
- OSTI ID:
- 5851573
- Journal Information:
- Biochemistry (Engl. Transl.); (United States), Journal Name: Biochemistry (Engl. Transl.); (United States) Vol. 51:2; ISSN BIORA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550301 -- Cytology-- Tracer Techniques
550401 -- Genetics-- Tracer Techniques
550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID HYDROLYSIS
ADENINES
AMINES
ANTIMETABOLITES
AROMATICS
AZAARENES
BIOCHEMISTRY
BIOSYNTHESIS
CELL DIFFERENTIATION
CEREALS
CHEMICAL REACTIONS
CHEMISTRY
CHROMATOGRAPHY
CYTOCHEMISTRY
DECOMPOSITION
DNA
DRUGS
ENZYME INHIBITORS
GRASS
HETEROCYCLIC COMPOUNDS
HYDROLYSIS
ISOTOPE APPLICATIONS
KININS
LABELLED COMPOUNDS
LABELLING
LYSIS
METABOLISM
METHYLATION
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
PLANT CELLS
PLANTS
POLYPEPTIDES
PROTEINS
PURINES
RADIOACTIVITY
RADIONUCLIDE KINETICS
RNA
SEEDS
SEPARATION PROCESSES
SOLVOLYSIS
SYNTHESIS
TRACER TECHNIQUES
TRANSCRIPTION
TRITIUM COMPOUNDS
WHEAT
550401 -- Genetics-- Tracer Techniques
550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID HYDROLYSIS
ADENINES
AMINES
ANTIMETABOLITES
AROMATICS
AZAARENES
BIOCHEMISTRY
BIOSYNTHESIS
CELL DIFFERENTIATION
CEREALS
CHEMICAL REACTIONS
CHEMISTRY
CHROMATOGRAPHY
CYTOCHEMISTRY
DECOMPOSITION
DNA
DRUGS
ENZYME INHIBITORS
GRASS
HETEROCYCLIC COMPOUNDS
HYDROLYSIS
ISOTOPE APPLICATIONS
KININS
LABELLED COMPOUNDS
LABELLING
LYSIS
METABOLISM
METHYLATION
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
PLANT CELLS
PLANTS
POLYPEPTIDES
PROTEINS
PURINES
RADIOACTIVITY
RADIONUCLIDE KINETICS
RNA
SEEDS
SEPARATION PROCESSES
SOLVOLYSIS
SYNTHESIS
TRACER TECHNIQUES
TRANSCRIPTION
TRITIUM COMPOUNDS
WHEAT