Rapid identification of cytokinins by an immunological method

Journal Article · Mon Apr 01 04:00:00 EST 1991 · Plant Physiology; (United States)

DOI:https://doi.org/10.1104/pp.95.4.1156· OSTI ID:5265135

Morris, R O; Jameson, P E; Morris, J W ^[1]; Laloue, M ^[2]

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1. Univ. of Missouri-Columbia (USA)
2. Centre Nationale de la Recherche Scientifique, Gif-sur-Yvette (France)

A method for rapid identification of bacterial cytokinins has been developed in which cultures are fed ({sup 3}H)adenine, the cytokinins (including, {sup 3}H-labeled cytokinins) are isolated by immunoaffinity chromatography, and analyzed by HPLC with on-line scintillation counting. Analysis of Agrobacterium tumefaciens strains showed that some produced primarily trans-zeatin, whereas others produced primarily trans-zeatin riboside. Pseudomonas syringae pv savastanoi produced mixtures of transzeatin, dihydrozeatin, 1{double prime}-methyl-trans-zeatin riboside, and other unknown cytokinin-like substances. Corynebacterium fascians, produced cis-zeatin, isopentenyladenine and isopentenyladenosine. The technique is designed for qualitative rather than quantitative studies and allows ready identification of bacterial cytokinins. It may also have utility in the study of plant cytokinins if adequate incorporation of label into cytokinin precursor pools can be achieved.

• OSTI ID: 5265135
• Journal Information: Plant Physiology; (United States), Journal Name: Plant Physiology; (United States) Vol. 95:4; ISSN 0032-0889; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
CHEMICAL ANALYSIS
CHROMATOGRAPHY
COUNTING TECHNIQUES
HORMONES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LIQUID COLUMN CHROMATOGRAPHY
MICROORGANISMS
PSEUDOMONAS
QUALITATIVE CHEMICAL ANALYSIS
SCINTILLATION COUNTING
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS