Induction of chromosomal aberrations in mouse zygotes by acrylamide treatment of male germ cells and their correlation with dominant lethality and heritable translocations

Journal Article · Wed Dec 31 04:00:00 EST 1997 · Environmental and Molecular Mutagenesis

DOI:https://doi.org/10.1002/(SICI)1098-2280(1997)30:4<410::AID-EM6>3.0.CO;2-M· OSTI ID:585132

Marchetti, F; Lowe, X; Wyrobek, A J ^[1]; Bishop, J ^[2]

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1. Lawrence Livermore National Lab., CA (United States)
2. National Institute of Environmental Health Sciences, Research Triangle Park, NC (United States)

The objectives of this research were: (1) to investigate the time course of the cytogenetic defects induced by acrylamide (AA) treatment (5 x 50 mg/kg) of male germ cells in first-cleavage zygote metaphases using PAINT/DAPI analysis, and (2) to characterize the correlation between chromosomal aberrations at first cleavage, dominant lethality, and heritable translocations. PAINT/DAPI analysis employs multicolor fluorescence in situ hybridization painting plus DAPI staining to detect both stable and unstable chromosomal aberrations at first-cleavage metaphase of the zygote. High levels of chromosomally defective zygotes were detected after mating at all postmeiotic stages (20-190-fold, P < 0.001). Early spermatozoa (6.5 d post-treatment) were the most sensitive, with 76% of the zygotes carrying cytogenetic defects. A significant 10-fold increase was also detected 27.5 d post-treatment, indicating that AA had a cytogenetic effect on meiotic stages. PAINT/DAPI analysis revealed that: (1) AA-induced chromosomal breaks occurred at random, and (2) the frequencies of symmetrical and asymmetrical exchanges were similar at all mating days, except 9.5 d after AA treatment, where significantly (P < 0.02) more asymmetrical aberrations were found. 33 refs., 5 figs., 4 tabs.

• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 585132
• Journal Information: Environmental and Molecular Mutagenesis, Journal Name: Environmental and Molecular Mutagenesis Journal Issue: 4 Vol. 30; ISSN 0893-6692; ISSN EMMUEG
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
ACRYLAMIDE
CHROMOSOMAL ABERRATIONS
GENETIC EFFECTS
MICE
ZYGOTES