1. alpha. ,25-dihydroxyvitamin D sub 3 regulates the expression of carbonic anhydrase II in nonerythroid avian bone marrow cells

Journal Article · Wed Aug 01 04:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.16.6470· OSTI ID:5850728

Billecocq, A; Emanuel, J R; Levenson, R; Baron, R ^[1]

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1. Yale Univ. School of Medicine, New Haven, CT (USA)

1{alpha},25-Dihydroxyvitamin D{sub 3} (1,25(OH){sub 2}D{sub 3}), the active metabolite of the steroid hormone vitamin D, is a potent regulator of macrophage and osteoclast differentiation. The mature osteoclast, unlike the circulating monocyte or the tissue macrophage, expresses high levels of carbonic anhydrase II (CAII). This enzyme generates protons and bicarbonate from water and carbon dioxide and is involved in bone resorption and acid-base regulation. To test whether 1,25(OH){sub 2}D{sub 3} could induce the differentiation of myelomonocytic precursors toward osteoclasts rather than macrophages, analyzed its effects on the expression of CAII in bone marrow cultures containing precursors common to both cell types. The expression of CAII was markedly increased by 1,25(OH){sub 2}D{sub 3} in a dose-and time-dependent manner. In bone marrow, this increase occurred at the mRNA and protein levels and was detectable as early as 24 hr after stimulation. 1,25(OH){sub 2}D{sub 3} was also found to induce CAII expression in a transformed myelomonocytic avian cell line. These results suggest that 1,25(OH){sub 2}D{sub 3} regulates the level at which myelomonocytic precursors express CAII, an enzyme that is involved in the function of the mature osteoclast.

• OSTI ID: 5850728
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 87:16; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ACID CARBONATES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BIOASSAY
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BONE MARROW CELLS
CARBON-OXYGEN LYASES
CARBONIC ANHYDRASE
CELL DIFFERENTIATION
CHEMISTRY
CHOLECALCIFEROL
CONNECTIVE TISSUE CELLS
CYTOCHEMISTRY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYME IMMUNOASSAY
ENZYMES
HYDRO-LYASES
IMMUNOASSAY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LYASES
MACROPHAGES
NUCLEI
ODD-EVEN NUCLEI
PHAGOCYTES
RADIOISOTOPES
SOMATIC CELLS
VITAMIN D
VITAMINS