Activation of the fructose 1,6-bisphosphatase gene by 1,25-dihydroxyvitamin D sub 3 during monocytic differentiation

Journal Article · Thu Sep 01 00:00:00 EDT 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.18.6904· OSTI ID:5600814

Solomon, D H; Raynal, M C; Tejwani, G A; Cayre, Y E ^[1]

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1. Cornell Univ. Graduate School of Medical Sciences, New York, NY (USA)

Cells from the human leukemia cell line HL-60 undergo terminal monocyte-like differentiation after exposure to either the active circulating form of vitamin D{sub 3}, 1,25-dihydroxyvitamin D{sub 3} (1,25-(OH){sub 2}D{sub 3}), or phorbol 12-myristate 13-acetate. Little is known about the genes that regulate monocytic differentiation. Using clonal variant cells of HL-60 origin, the authors constructed a cDNA library enriched for genes that are induced by 1,25-(OH){sub 2}D{sub 3}. They report that in HL-60, the fructose 1,6-bisphosphatase gene is activated during 1,25-(OH){sub 2}D{sub 3}-induced monocytic differentiation. This gene encodes two closely related mRNAs; one, activated by 1,25-(OH){sub 2}D{sub 3} at an early stage of HL-60 differentiation, encodes a protein that has homology to mammalian FBPase, a key enzyme in gluconeogenesis, although it does not exhibit its classical enzymatic activity. A second mRNA is activated by 1,25-(OH){sub 2}D{sub 3} mainly in peripheral blood monocytes. This mRNA is present in kidney as a unique transcript and encodes a protein with FBPase activity. The data also show that this FBPase-encoding mRNA can be activated during monocytic maturation since it was detected in human alveolar macrophages.

• OSTI ID: 5600814
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 85:18; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CELL DIFFERENTIATION
CHOLECALCIFEROL
DAYS LIVING RADIOISOTOPES
DNA
ELECTROPHORESIS
ENZYME INDUCTION
ENZYMES
ESTERASES
ESTERS
GENE REGULATION
GENES
HYBRIDIZATION
HYDROLASES
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
MATERIALS
MESSENGER-RNA
MONOCYTES
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHORBOL ESTERS
PHOSPHATASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RECOMBINANT DNA
RNA
TUMOR CELLS
VITAMIN D
VITAMINS