Regulation of 1,25-dihydroxyvitamin D sub 3 receptors in human promyelocytic leukemic cells, HL-60

Thesis/Dissertation · Sat Dec 31 23:00:00 EST 1988

OSTI ID:6529400

Lee, Y

The regulation of 1,25-dihydroxyvitamin D{sub 3} (1,25-(OH){sub 2}D{sub 3}) was investigated in human target cells using human promyelocytic leukemic cell line, HL-60, as a model system. Receptors for 1,25-(OH){sub 2}D{sub 3} in intact HL-60 cells were saturated with ({sup 3}H)1,25-(OH){sub 2}D{sub 3} within 1 h after hormone addition. Continuous exposure of cells to hormone for longer times resulted in an apparent up-regulation which was maximal between 8-16 h after hormone addition. This was followed by an apparent down-regulation which could be observed by 24 h. The immunoblotting assays indicated that an up-regulation resulted from actual increases in receptor protein and a down-regulation from a decrease in receptor protein and a loss of binding ability of receptors. The monocytic inducers of HL-60, 1,25-(OH){sub 2}D{sub 3} and phorbol 12-myristate 13-acetate (PMA) could regulate the level of receptors. However, the granulocytic inducers of HL-60 cells, retinoic acid and dimethyl sulfoxide failed to regulate the specific ({sup 3}H)1,25-(OH){sub 2}D{sub 3} binding.

• Research Organization: Wisconsin Univ., Madison, WI (USA)
• OSTI ID: 6529400
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CARCINOGENS
CELL DIFFERENTIATION
CHROMATOGRAPHY
DISEASES
ESTERS
HEMIC DISEASES
HYDROGEN COMPOUNDS
IMMUNE SYSTEM DISEASES
ISOTOPE APPLICATIONS
KINETICS
LEUKEMIA
MAMMALS
MAN
MEMBRANE PROTEINS
NEOPLASMS
ORGANIC COMPOUNDS
PHORBOL ESTERS
PRIMATES
PROTEINS
REACTION KINETICS
RECEPTORS
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
VERTEBRATES
VITAMIN D
VITAMINS