Translocation of labelled sucrose: A student exercise

Conference · Tue May 01 04:00:00 EDT 1990 · Plant Physiology, Supplement; (USA)

OSTI ID:5850060

Reiss, C ^[1]

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1. Cornell Univ., Ithaca, NY (USA)

Photosynthetic carbohydrates from the leaves are exported through the phloem to growing tips, roots, flowers and fruits. If sucrose labelled with {sup 14}C is applied to the leaves of bean plants, the pathway for sugar movement may be readily observed by autoradiography. Students apply the labelled sucrose during class time and return the next day to press their plants. During the next class, the pressed and dried plants are placed against X-ray film and left in the dark for four weeks. the film is then developed, examined for presence of label and compared to the pressed plants. Source to sink movement is clearly illustrated and information about the mechanism of phloem transport and loading is gained through experimental treatments, which include blocking the phloem pathway and inhibiting energy production.

• OSTI ID: 5850060
• Report Number(s): CONF-9007196--
• Conference Information: Journal Name: Plant Physiology, Supplement; (USA) Journal Volume: 93:1
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
CARBON 14 COMPOUNDS
CHEMICAL REACTIONS
DATA
EDUCATIONAL TOOLS
EXPERIMENTAL DATA
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEAVES
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
NUMERICAL DATA
PHASEOLUS
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PLANTS
SYNTHESIS
TRACER TECHNIQUES