Effect of X irradiation on a heterotransplanted human colonic carcinoma before and after a change in the cellular DNA content

Journal Article · Tue Feb 01 04:00:00 EST 1983 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575985· OSTI ID:5849216

Spang-Thomsen, M ^[1]; Vindelov, L L; Nielsen, A

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1. Finsen Inst., Copenhagen, Denmark

A spontaneous change of cellular DNA content occurred in a hyperdiploid human colonic carcinoma grown in nude mice. After the change to hyperpentaploidy the tumor was exposed to single-dose X irradiation, and the effects on growth curves and on the cell cycle, determined by flow cytometric DNA analysis (FCM), were compared to results obtained with the tumor prior to the evolutionary event. The results showed that the radiation effects on growth rate and on cell kinetics had changed after the change in cellular DNA content. In the hyperpentaploid tumor the irradiation had no effect on the regrowth rate, whereas in the hyperdiploid tumor the postirradiation growth rate had decreased. As a consequence of the different effect on the regrowth rate of the tumors the growth delay was inadequate as a parameter for comparing the radiosensitivity. In the hyperpentaploid tumor the irradiation induced a partial synchronization of accumulated cells, whereas no synchronization effect was found in the hyperdiploid tumor. The redistribution time was 8-10 days for both tumors. The results indicate that clonal evolution may affect radiosensitivity, and that FCM analysis may prove to be a valuable method to provide rapid information on cellular synchronization and on redistribution time.

• OSTI ID: 5849216
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Journal Issue: 2 Vol. 93:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARCINOMAS
CELL CYCLE
CELL FLOW SYSTEMS
DATA
DISEASES
DNA
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
GROWTH
INFORMATION
IONIZING RADIATIONS
KINETICS
MAMMALS
MAN
MICE
NEOPLASMS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
PLOIDY
PRIMATES
RADIATION EFFECTS
RADIATIONS
RODENTS
VERTEBRATES
X RADIATION