Scintillation fluid shortens exposure times in autoradiography
Journal Article
·
· J. Histochem. Cytochem.; (United States)
Premixed, commercially available scintillation fluids were used to reduce exposure time of tritium (/sup 3/H) and iodine-125 (/sup 125/I)-labeled whole cells, and of /sup 3/H-labeled Giemsa-banded chromosome preparations. Emulsion-coated slides were dipped into scintillator for no longer than 2 min and exposed in the dark at 4 degrees C. Maximal values for percentage of human diploid cell (WI-38) nuclei labeled with /sup 3/H-thymidine of moderate specific activity were obtained in 12 h. Without scintillator the exposure time was 4 days. Exposure time for cells labeled with /sup 125/I-serum was reduced from over 90 days to 14 days. The shortened exposure time for banded chromosomes permitted successful prestaining with Giemsa, a sequence that is not possible without scintillator.
- Research Organization:
- Wistar Inst. of Anatomy and Biology, Philadelphia, PA
- OSTI ID:
- 5842542
- Journal Information:
- J. Histochem. Cytochem.; (United States), Journal Name: J. Histochem. Cytochem.; (United States) Vol. 27:10; ISSN JHCYA
- Country of Publication:
- United States
- Language:
- English
Similar Records
High-speed scintillation autoradiography
Differential Autoradiography of Tritium and Another β-Emitter by a Double Stripping Film Technique
High-speed electron microscope autoradiographic studies of diffusible compounds
Journal Article
·
Thu Dec 11 23:00:00 EST 1975
· Science, v. 190, no. 4219, pp. 1093-1095
·
OSTI ID:4145193
Differential Autoradiography of Tritium and Another β-Emitter by a Double Stripping Film Technique
Journal Article
·
Sat Aug 04 00:00:00 EDT 1962
· Nature (London)
·
OSTI ID:4797070
High-speed electron microscope autoradiographic studies of diffusible compounds
Journal Article
·
Wed Dec 31 23:00:00 EST 1980
· J. Histochem. Cytochem.; (United States)
·
OSTI ID:5673848
Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOMEDICAL RADIOGRAPHY
BODY
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
ELECTRON CAPTURE RADIOISOTOPES
EMBRYOS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LUNGS
MEDICINE
NUCLEAR MEDICINE
NUCLEI
ODD-EVEN NUCLEI
OPTIMIZATION
ORGANS
RADIOISOTOPE SCANNING
RADIOISOTOPES
RADIOLOGY
RESPIRATORY SYSTEM
SCINTISCANNING
TRITIUM COMPOUNDS
62 RADIOLOGY AND NUCLEAR MEDICINE
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOMEDICAL RADIOGRAPHY
BODY
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
ELECTRON CAPTURE RADIOISOTOPES
EMBRYOS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LUNGS
MEDICINE
NUCLEAR MEDICINE
NUCLEI
ODD-EVEN NUCLEI
OPTIMIZATION
ORGANS
RADIOISOTOPE SCANNING
RADIOISOTOPES
RADIOLOGY
RESPIRATORY SYSTEM
SCINTISCANNING
TRITIUM COMPOUNDS