Insulin-like growth factor-II (IGF II) receptor from rat brain is of lower apparent molecular weight than the IGF II receptor from rat liver

Journal Article · Thu Oct 01 00:00:00 EDT 1987 · Endocrinology; (United States)

DOI:https://doi.org/10.1210/endo-121-4-1306· OSTI ID:5842236

McElduff, A; Poronnik, P; Baxter, R C

The binding subunits of the insulin and insulin-like growth factor-I (IGF I) receptors from rat brain are of lower molecular weight than the corresponding receptor in rat liver, possibly due to variations in sialic acid content. We have compared the IGF II receptor from rat brain and rat liver. The brain receptor is of smaller apparent mol wt (about 10 K) on sodium dodecyl sulfate polyacrylamide gel electrophoresis. This size difference is independent of ligand binding as it persists in iodinated and specifically immunoprecipitated receptors. From studies of wheat germ agglutinin binding and the effect of neuraminidase on receptor mobility, we conclude that this difference is not simply due to variations in sialic acid content. Treatment with endoglycosidase F results in reduction in the molecular size of both liver and brain receptors and after this treatment the aglycoreceptors are of similar size. We conclude that in rat brain tissue the IGF II receptor like the binding subunits of the insulin and IGF I receptors is of lower molecular size than the corresponding receptors in rat liver. This difference is due to differences in N-linked glycosylation.

• Research Organization: Royal North Shore Hospital, Sydney, Australia
• OSTI ID: 5842236
• Journal Information: Endocrinology; (United States), Vol. 121:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GROWTH FACTORS
RECEPTORS
INSULIN
BIOCHEMICAL REACTION KINETICS
AGGLUTININS
BRAIN
COMPARATIVE EVALUATIONS
ELECTROPHORESIS
GLUCOSIDASE
IODINE ISOTOPES
LIVER
MOLECULAR WEIGHT
RATS
TRACER TECHNIQUES
ANIMALS
ANTIBODIES
BODY
CENTRAL NERVOUS SYSTEM
DIGESTIVE SYSTEM
ENZYMES
GLANDS
GLYCOSYL HYDROLASES
HORMONES
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MITOGENS
NERVOUS SYSTEM
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PROTEINS
REACTION KINETICS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques